Evidence map›Paper›PMID 41276478›Full record

ArticleJournal of Zhejiang University. Science. B2025

Polarizing macrophages derived from human THP-1 cells in vitro: methods and protocols.

Pengfei Li, Lin Chen, Wei Yuan, Xingqiang Li, Xuesong Feng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of Zhejiang University. Science. B, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Pengfei LiLaboratory of Animal Center, Medical Experiment Center, Shaanxi University of Chinese Medicine, Xianyang 712046, China.
Lin ChenDepartment of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Wei YuanLaboratory of Animal Center, Medical Experiment Center, Shaanxi University of Chinese Medicine, Xianyang 712046, China.
Xingqiang LiBasic Medical Academy, Shaanxi University of Chinese Medicine, Xianyang 712046, China.
Xuesong FengLaboratory of Animal Center, Medical Experiment Center, Shaanxi University of Chinese Medicine, Xianyang 712046, China. 2111051@sntcm.edu.cn.

Funding

the Graduate Student Innovative Practical Ability Improvement Project CXSJ202302the Shaanxi Key Laboratory of Research on TCM Physical Constitution and Diseases Prevention and Treatment KF202311the Shaanxi University of Chinese Medicine-National Natural Science Foundation Cultivation Project 2023GP16
6 · The paper itself

Abstract

Macrophages derived from the human THP-1 cell line have been widely used as substitutes for primary macrophages in various macrophage-related studies. However, difficulties still exist in establishing THP-1 macrophage models. This research presents techniques for generating different phenotypes of activated macrophages derived from THP-1 cells by introducing specific stimuli and provides some potential markers to confirm each type of activated macrophage. It is hoped to provide novel and useful methods for scientific research and to help researchers explore this field more intuitively and effectively.

Indexed as

Cell Culture TechniquesCell PolarityMacrophagesCell DifferentiationCell LineHumansMacrophage ActivationPhenotypeTHP-1 Cells

Identifiers

PMID41276478
PMCPMC12640744

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.