ArticleBMC cancer2025
Single-cell RNA sequencing reveals neutrophil differentiation-related genes for immunotherapy response prediction in colorectal cancer.
Article in BMC cancer, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Reshaping the colorectal cancer immune microenvironment: insights from single-cell and spatial omics.Cancer cell international · 2026Review
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Authors and funding
10 authors.
Funding
Abstract
backgroundImmunotherapy has demonstrated promising results in treating colorectal cancer (CRC), yet not all patients benefit equally, highlighting the need for predictive biomarkers and additional adjuvant. Neutrophils, pivotal in immune regulation and inflammation, remain underexplored in the context of CRC immunotherapy, holding the potential targets for improving immunotherapy efficacy.
methodsSingle-cell RNA sequencing data from 19 CRC patients, including immunotherapy-treated individuals and controls, were analyzed. Genes associated with neutrophil differentiation, termed Neutrophil Differentiation-Related Genes (NDRGs), were identified through trajectory analysis and refined to nine genes (TMBIM6, CTSS, CYCS, DDX3X, DYNLL1, LGALS1, GANI2, RPS29, and TUBA1A) using Elastic Net. Drug screening was performed to identify potential therapeutic agents targeting NDRGs.
resultsImmunotherapy induced a shift in neutrophil composition, marked by a reduction in inflammatory neutrophils and an increase in immune neutrophils. The nine NDRGs were successfully validated and utilized to develop a predictive model for immunotherapy response. Drug screening identified Ivermectin as a potential therapeutic agent targeting NDRGs.
conclusionsThis study highlights the dynamic role of neutrophils in the CRC immune microenvironment, their potential as biomarkers for immunotherapy response, and their utility as therapeutic targets to improve immunotherapy outcomes.
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