Application of Allium cepa Test for Genetic Toxicology.

Anita Mukherjee, Manosij Ghosh, Ritesh Banerjee

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Anita MukherjeeCell Biology and Genetic Toxicology Laboratory, Centre of Advanced Study, Department of Botany, University of Calcutta, Kolkata, India. ambot@caluniv.ac.in.

Manosij GhoshCell Biology and Genetic Toxicology Laboratory, Centre of Advanced Study, Department of Botany, University of Calcutta, Kolkata, India.

Ritesh BanerjeeCell Biology and Genetic Toxicology Laboratory, Centre of Advanced Study, Department of Botany, University of Calcutta, Kolkata, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Allium cepa (A. cepa) root tip assay is considered an excellent genetic model for genotoxicity screening and monitoring of environmental mutagens. The assay is highly versatile for genetic endpoints, inexpensive, quick, and easy to use. It can be used with a range of environmental conditions, pH, and temperature, and does not require any ethical regulations as required for animal models and cell lines. In this chapter, we describe the modifications of the assay to analyze different endpoints of genotoxicity. They include the most commonly used chromosome aberrations, the mitotic index to evaluate cytotoxicity, and the analysis of micronuclei to verify the mutagenicity of different chemicals. Moreover, the A. cepa test system also provides a sensitive method for the evaluation of DNA damage and DNA repair capacity at the single-cell level by the Comet assay.

Indexed as

MutagensOnionsChromosome AberrationsComet AssayDNA DamageDNA RepairMicronucleus TestsMitotic IndexMutagenicity TestsMutagensChromosome aberrationsComet assayDNA damageMicronucleus assayMitotic index

Identifiers

PMID41273695

DOI10.1016/j.ecoleng.2014.10.011

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.