Evidence map›Paper›PMID 41273679›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Erythrocyte-Based Pig-a Gene Mutation Assay.

Jeffrey C Bemis, Nikki E Hall, Stephen D Dertinger

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jeffrey C BemisLitron Laboratories, Rochester, NY, USA. jbemis@litronlabs.com.
Nikki E HallLitron Laboratories, Rochester, NY, USA.
Stephen D DertingerLitron Laboratories, Rochester, NY, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In addition to chromosomal damage, assessment of gene mutation is an important part of genotoxicity testing employed during preclinical safety testing. The Pig-a gene mutation assay is based on the loss of function of the Pig-a gene, which results in a lack of cell surface expression of specific proteins that are targeted to the surface by glycosylphosphatidylinositol (GPI) anchors. This cell surface phenotype is readily assessed by flow cytometric analysis of red blood cells. This unit describes a procedure for the collection, processing, and analysis of peripheral blood samples using materials supplied in MutaFlow

Indexed as

ErythrocytesMembrane ProteinsMutationAnimalsFlow CytometryGlycosylphosphatidylinositolsHumansMutagenicity TestsGlycosylphosphatidylinositolsMembrane Proteinsphosphatidylinositol glycan-class A proteinEndogenous mutationErythrocytesFlow cytometryGenotoxicityGlycosylphosphatidylinositol (GPI) anchorHigh throughputImmunomagnetic separationPig-aReticulocytes

Identifiers

PMID41273679

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.