Evidence map›Paper›PMID 41273654›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Lentiviral Vector-Mediated Gene Delivery for iPSC-Related Neuroscience Research.

Taro Okunomiya, Keiko Imamura, Taku Kashiyama, Takashi Sakurai, Hiroyuki Hioki, Haruhisa Inoue

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Taro OkunomiyaCenter for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto, Japan.
Keiko ImamuraCenter for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto, Japan.
Taku KashiyamaDepartment of Cellular and Molecular Pharmacology, Juntendo University Graduate School of Medicine, Tokyo, Japan.
Takashi SakuraiDepartment of Cellular and Molecular Pharmacology, Juntendo University Graduate School of Medicine, Tokyo, Japan.
Hiroyuki HiokiDepartment of Neuroanatomy, Juntendo University Graduate School of Medicine, Tokyo, Japan.
Haruhisa InoueCenter for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto, Japan. haruhisa@cira.kyoto-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The induced pluripotent stem cell (iPSC) technology provides human neural cells unprecedented material for neuroscience research. Lentiviral vectors have the advantage of being able to transfer genes into nondividing cells, which is useful for the identification and purification of specific neurons generated from human iPSCs, as well as for analysis of their function. Furthermore, the lentiviral vector has long-term expression and large gene capacity. This chapter describes protocols for lentiviral generation, labeling of iPSC-derived motor neurons with fluorescent proteins, and imaging of synaptic function.

Indexed as

Genetic VectorsGene Transfer TechniquesInduced Pluripotent Stem CellsLentivirusNeurosciencesCell DifferentiationHumansMotor NeuronsTransduction, GeneticGene deliveryInduced pluripotent stem cellsLentiviral vectorNondividing cellsPurificationSynapse imaging

Identifiers

PMID41273654

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.