Selective Chemogenetic Blockade of Cerebello-Rubral Pathway by Double-Virus Infection Method Using Lentivirus and Adeno-Associated Virus in Rats.

Hideki Hida, Shinya Ueno

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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4 · The record

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5 · Who and what money

Authors and funding

2 authors.

Hideki HidaDepartment of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. hhida@med.nagoya-cu.ac.jp.

Shinya UenoDepartment of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Double-virus infection method of lentivirus and adeno-associated virus was used to reveal the contribution of cerebellum in rehabilitative training after intracerebral hemorrhage (ICH) in rats. Chemogenetic blockade of cerebello-rubral pathway was achieved by the injection of a lentiviral vector pseudotyped with fusion glycoprotein type E and carrying Cre recombinase into the red nucleus and an adeno-associated viral vector encoding hM4D(Gi)-mCherry in the double-floxed, inverted orientation into the cerebellar lateral nucleus. This silencing system induced by clozapine-N-oxide significantly reduced the success rate of forelimb reaching test that is improved by forced-limb use after ICH. As a good application example of loss-of-function technique, the protocol of our experiment by double-virus infection method will be explained in detail.

Indexed as

CerebellumDependovirusLentivirusAnimalsChemogeneticsClozapineGenetic VectorsIntegrasesMaleRatsRed NucleusClozapineclozapine N-oxideIntegrasesCerebello-rubral pathwayDouble-virus infection methodIntracerebral hemorrhageMotor control systemRatRehabilitationSelective pathway silencing

Identifiers

PMID41273652

DOI10.1016/j.bbr.2015.02.022

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.