ArticleMethods in molecular biology (Clifton, N.J.)2026
Lentivirus-Mediated Measurement of Transcription Factor Activity in the Brain.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Transcription factors (TFs) play a pivotal role in the plastic changes of the brain. Measuring TF activity in vivo and uncovering changes under various physiological and pathophysiological conditions provide valuable insights into brain mechanisms. This protocol outlines a detailed procedure for measuring TF activity in the brain by using lentiviral vector (LV)-based TF reporters. The protocol covers the preparation of LV, injection of the vector into the mouse brain, mRNA processing from the tissue, and quantitative PCR-based calculation of TF activity.
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