Evidence map›Paper›PMID 41273108›Full record

ArticleAnnals of medicine2025

Higher levels of neutrophil extracellular traps in mucoid middle ear effusion obtained from otitis media with effusion in children.

Lining Guo, Tiewei Li, Qingchuan Duan, Yongtao Duan, Wei Liu, Ning Ma, Shanshan Liu, Xiaoxu Wang, Shengnan Zhang, Enxia Tian and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Lining GuoDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.ORCID 0000-0002-3660-4864
Tiewei LiDepartment of Clinical Laboratory, Children's Hospital Affiliated to Zhengzhou University, Zhengzhou Key Laboratory of Children's Infection and Immunity, Zhengzhou, China.ORCID 0000-0003-4890-1134
Qingchuan DuanDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.
Yongtao DuanHenan Children's Hospital, Zhengzhou Children's Hospital, Henan Key Laboratory of Children's Genetics and Metabolic Diseases, Henan Children's Neurodevelopment Engineering Research Center, Children's Hospital Affiliated to Zhengzhou University, Zhengzhou, China.
Wei LiuDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.
Ning MaDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.
Shanshan LiuDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.
Xiaoxu WangDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.
Shengnan ZhangDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.
Enxia TianDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.
Xin NiDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.
Jie ZhangDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.ORCID 0000-0002-1621-7779

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe etiology of otitis media with effusion (OME) is multifactorial and complex, with increasing evidence implicating bacterial infection as a potential contributor. Neutrophil extracellular traps (NETs) have been identified as key participants in the pathophysiological processes of various infectious diseases. However, to date, no studies have quantitatively assessed NETs in OME or explored its role in the middle ear.

objectiveThis study aims to quantitatively assess NETs and mucins in different types of middle ear effusion (MEE) in children with OME, investigate the correlations between NETs, mucins, and clinical phenotypes, and explore the potential role of NETs in OME.

methodsWe included fifty-five pediatric OME patients underwent tympanostomy tube insertion (March-November 2024). Seventy MEE specimens were collected for detection and analysis. General demographics, disease duration, hearing thresholds and blood cell levels were documented. All samples were characterized into two main types (serous and mucoid) by the same surgeon based on their appearance and viscosity. NET-related biomarkers (citrullinated histone H3 [CitH3], double-stranded DNA [dsDNA], myeloperoxidase-DNA complex [MPO-DNA], and Nucleosome), along with mucins (mucin5AC [MUC5AC] and mucin5B [MUC5B]) were detected using enzyme-linked immunosorbent assay (ELISA), modified ELISA or fluorometry.

resultsSubjects in the mucoid group demonstrated more severe hearing impairment than those in the serous group (

conclusionsNET-related biomarkers were significantly increased in mucoid effusions with a notable positive correlation to MUC5AC, MUC5B, and hearing levels.

Indexed as

Extracellular TrapsOtitis Media with EffusionBiomarkersChildChild, PreschoolEar, MiddleFemaleHumansInfantMaleMiddle Ear VentilationMucinsNeutrophilsBiomarkersMucinschildreninfectionmucinNeutrophil extracellular trapsotitis media with effusion

Identifiers

PMID41273108
PMCPMC12642906

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.