ArticleBMC research notes2025
A fluorescent biosensor enables high-sensitivity quantification of hemolymph trehalose in individual aphids.
Article in BMC research notes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
objectiveTrehalose is a major hemolymph sugar in aphids; however, its accurate quantification at the individual level has been technically challenging because of the small body size of aphids. To quantify hemolymph trehalose at the single-insect level in aphids using a GFP-based fluorescent biosensor (Tre‑C04), benchmark it against high-performance liquid chromatography (HPLC), and examine species- and wing-morph differences.
resultsHPLC of pooled adult Acyrthosiphon pisum hemolymph measured 218.9 ± 34.4 mM trehalose, while Tre‑C04 quantified 183.5-261.7 mM in individual adults (mean 227.3 ± 10.0 mM); the two methods did not differ significantly. Across nine species, single-insect measurements clustered near ~ 200 mM (191.7-249.2 mM), and we did not reproduce previously reported values > 600 mM in Aphis gossypii or Myzus persicae; pooling required by
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