ArticleParasites & vectors2025
Novel gp60 subtypes and genetic diversity of Cryptosporidium felis in domestic and stray cats from Central Europe.
Article in Parasites & vectors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundCryptosporidium felis, a host-specific protozoan with possible zoonotic potential, is a common parasite of cats (Felis catus). To date, there have been few studies on the molecular subtyping of C. felis worldwide, and no have been conducted in Central Europe. The aim of this study was to analyse the prevalence and genetic variability of C. felis in domestic and stray cats in Central Europe, particularly in the Czech Republic, Poland and Slovakia.
methodsFaecal samples were collected from domestic and stray cats. The presence of Cryptosporidium spp. was analysed by light microscopy with aniline-carbol-methyl violet staining and polymerase chain reaction (PCR)/sequencing of the small subunit rRNA gene (18S rDNA). All PCR-positive samples were further subtyped using PCR and sequencing of the 60 kDa glycoprotein gene (gp60). A chi-square test and odds ratio (OR) analysis were used to compare infection rates and assess the risk of C. felis infection between domestic and stray cats.
resultsA total of 711 faecal samples were collected - 350 from domestic cats and 361 from stray cats. The overall infection rate of Cryptosporidium spp. was 4.5% (32/711), with stray cats being significantly more frequently infected (6.7%) than domestic cats (2.3%). Oocysts of Cryptosporidium spp. were not detected microscopically in any of the samples. There were no significant differences between the infection rates in the three countries. All isolates were identified as C. felis, and analysis of the gp60 gene revealed five different subtypes, all belonging to the XIXa subtype family of C. felis. These subtypes formed five well-supported phylogenetic clusters, none of which had been previously reported worldwide. Only one subtype was found in domestic cats, whereas all five subtypes were found in stray cats. The subtypes identified in stray cats showed a clear geographical distribution in the study region.
conclusionsThe results of this study extend our knowledge of the genetic variability of C. felis and indicate a possible geographical distribution of the detected subtypes. The significance of the observed genetic variability in terms of geographical distribution, host specificity and zoonotic potential remains unclear and requires further investigation.
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