Evidence map›Paper›PMID 41272458›Full record

ArticleBMC plant biology2025

Loci and natural alleles for leaf length and width revealed by a genome wide association study in adzuki bean.

Xiaocao Wu, Yuxin Wen, Kun Wu, Runzhi Li, Xingen Li, Xuehan Zhang, Liwei Chu, Yongqiang Li, Kai Yang, Ping Wan and 2 more

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Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Xiaocao Wu *College of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China.
Yuxin Wen *College of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China.
Kun Wu *State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Runzhi Li *College of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China.
Xingen LiCollege of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China.
Xuehan ZhangCollege of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China.
Liwei ChuCollege of Life and Health, Dalian University, Dalian, Liaoning, China.
Yongqiang LiCollege of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China.
Kai YangCollege of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China.
Ping WanCollege of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China. pingwan3@163.com.
Jianping YuCollege of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China. jpyu@bua.edu.cn.
Bo ZhaoCollege of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China. zhaobobac@sohu.com.

Funding

Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture KFKT-2025011Beijing University of Agriculture Young Faculty Research Innovation Enhancement Program QJKC-2022050
6 · The paper itself

Abstract

backgroundLeaf size and shape are crucial for photosynthesis and consequently, yield in adzuki beans (Vigna angularis L.). This study utilized 321 adzuki bean germplasm accessions (100 wild/semi-wild and 221 cultivated varieties) to analyze leaf length (LL) and leaf width (LW) phenotypes across two locations (Beijing and Anhui, China) from 2014 to 2017. Genome-wide association studies (GWAS) were then employed to elucidate the underlying genetic mechanisms.

resultsThe results demonstrated that cultivated adzuki beans exhibited significantly larger leaves (LL: 6.64-10.84 cm; LW: 5.22-9.23 cm) compared to wild populations (LL: 5.65-6.21 cm; LW: 4.52-4.83 cm). Improved cultivars consistently displayed greater leaf width across multiple growing seasons than landraces, highlighting the effects of domestication and breeding improvement on leaf morphology. A significant geographic effect was also observed, with adzuki beans grown in Beijing generally exhibiting larger leaves than those grown in Anhui, indicating the influence of environmental factors. GWAS identified 100 quantitative trait loci (QTLs) associated with leaf morphology across the entire population (72 for LL and 28 for LW), distributed across chromosomes 1-11, with chromosome 4 showing the highest number of QTLs (16). Eight candidate genes were selected based on gene annotation, cross-species homology comparisons, and haplotype analysis. For example, Vigan01g073100, a candidate gene for LL, encodes a receptor protein kinase involved in regulating cell division and elongation, thus impacting organ development. Vigan07g22880, a candidate gene for LW, encodes an auxin efflux carrier, and its haplotype variation (Val-Ala substitution) led to significantly wider leaves in cultivated populations compared to wild populations. Haplotype analysis revealed genetic differentiation of these genes between cultivated and wild subpopulations, with some advantageous haplotypes (e.g., Hap.1 of Vigan02g058600) showing significant associations with leaf morphology phenotypes.

conclusionsThis study presents the first comprehensive analysis of the genetic basis of leaf morphology in adzuki beans, identifying key candidate genes involved in cell proliferation, auxin transport, and transcription regulation. These findings provide valuable genetic resources and theoretical support for understanding the molecular mechanisms of leaf development and improving leaf morphology in adzuki bean breeding programs.

Indexed as

Plant LeavesQuantitative Trait LociVignaAllelesChinaGenome-Wide Association StudyPhenotypePolymorphism, Single NucleotideAdzuki beansGWASHaplotype analysisLeaf lengthLeaf width

Identifiers

PMID41272458
PMCPMC12640002

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LicenceCC BY-NC-ND
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.