Noninvasive monitoring and prediction of cell count of Saccharomyces suspensions using ultrasonic measurements and artificial neural networks.

The dynamic nature of biological suspensions during fermentation requires continuous monitoring. In Saccharomyces suspensions, cell count directly influences biomass production and is an indicator of metabolic efficiency. However, established methods to monitor cell count require sampling, are invasive, or are restricted by detection limits. In contrast, ultrasound techniques are noninvasive and provide real-time feedback. In this study, ultrasound was used to analyze yeast suspensions with increasing concentrations (0.0-1.0 wt%) from three Saccharomyces strains, each assessed at three wort concentrations (10, 12, and 14 wt% extract). The collected analytical data, consisting of manually determined cell count and nine ultrasound-derived features, were used to develop separate artificial neural network (ANN) models for cell count prediction. Three strain-specific regression models were initially developed; their evaluation metrics confirmed that the selected ANN architecture reliably predicted cell count for each strain (R