Evidence map›Paper›PMID 41271770›Full record

ArticleNPJ science of food2025

Cloning, expression and functional characterization of prepared bovine, salmon, and cod basic fibroblast growth factor-2.

S Skrivergaard, M E Pedersen, A Holck, A Fagerlund, L Axelsson, N T Solberg, M K Rasmussen, J F Young, M Therkildsen, S B Rønning

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in NPJ science of food, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

S SkrivergaardDepartment of Food Science, Aarhus University, Aarhus, Denmark.
M E PedersenNofima AS, Raw Materials and Optimization, Ås, Norway. mona.pedersen@nofima.no.
A HolckNofima AS, Food Safety and Quality, Ås, Norway.
A FagerlundNofima AS, Food Safety and Quality, Ås, Norway.
L AxelssonNofima AS, Food Safety and Quality, Ås, Norway.
N T SolbergNofima AS, Raw Materials and Optimization, Ås, Norway.
M K RasmussenDepartment of Food Science, Aarhus University, Aarhus, Denmark.
J F YoungDepartment of Food Science, Aarhus University, Aarhus, Denmark.
M TherkildsenDepartment of Food Science, Aarhus University, Aarhus, Denmark.
S B RønningNofima AS, Raw Materials and Optimization, Ås, Norway.

Funding

Landbrugsstyrelsen 33010-NIFA-19-716Norwegian Fund for Research Fees for Agricultural Products (FFL) 314111
6 · The paper itself

Abstract

Growth factors (GFs) contribute to over 90% of the total expenses of medium costs. In this study, a codon-optimized gene encoding bovine, salmon, and cod FGF-2 was cloned into an E. coli expression vector. The bioactivity of the recombinant FGF-2 was tested in serum-free medium, demonstrating its ability to support MuSC proliferation and signaling pathways, ERK, and p38. Using serum-free medium with fetuin and Insulin-Transferrin-Selenium (ITS), all three in-house-produced recombinant FGF-2 variants showed positive effects on MuSC proliferation. Notably, bovine FGF-2 performed better using low concentrations (2 ng/mL) than the salmon and cod FGF-2, which required higher concentrations (17.5-70 ng/mL) to achieve the same effect on the cells. A synergistic relationship between bovine FGF-2 and the p38 inhibitor was revealed. These findings suggest that the medium cost from FGF-2 can be significantly reduced with optimized production and application strategies, supporting the large-scale viability of CM production.

Identifiers

PMID41271770
PMCPMC12639020

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.