Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registry
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Ashna NagpalDepartment of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Matthew A SchaichDepartment of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Tyler M WeaverDepartment of Biochemistry and Molecular Genetics, University of Virginia School of Medicine and University of Virginia Comprehensive Cancer Center, Charlottesville, VA, USA.
Vera RoginskayaUPMC-Hillman Cancer Center, Pittsburgh, PA, USA.
Annahita SallmyrUniversity of New Mexico Comprehensive Cancer Center and the Departments of Internal Medicine, and Molecular Genetics & Microbiology, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
Liam P LearyUPMC-Hillman Cancer Center, Pittsburgh, PA, USA.
Bret D FreudenthalDepartment of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS, USA.ORCID http://orcid.org/0000-0003-1449-4710
Alan E TomkinsonUniversity of New Mexico Comprehensive Cancer Center and the Departments of Internal Medicine, and Molecular Genetics & Microbiology, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.ORCID http://orcid.org/0000-0002-2671-1711
Bennett Van HoutenDepartment of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. vanhoutenb@upmc.edu.ORCID http://orcid.org/0000-0002-4009-2478
Funding
VECTOR CORE FACILITYP30CA047904 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Jessie Nedrow · 1988 to 2026
$158.0M
Transcription-Coupled & Replication-Associated Excision RepairP01CA092584 · NCI · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI John A. Tainer · 2001 to 2026
$89.6M
WOMEN'S CANCERS RESEARCH PROGRAMP30CA118100 · NCI · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI Yolanda Sanchez · 2005 to 2026
$57.1M
Roles of LIG3 and XRCC1 genes in genome stability.R01ES012512 · NIEHS · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI Alan E Tomkinson · 2004 to 2026
$6.3M
Watching cooperative interactions between base and nucleotide excision repair proteinsR35ES031638 · NIEHS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Bennett Van Houten · 2020 to 2026
$6.2M
Supplement to NIH Award 2 R35GM128562-06R35GM128562 · NIGMS · UNIVERSITY OF KANSAS MEDICAL CENTER · PI Bret D Freudenthal · 2018 to 2026
$4.2M
Lumicks C-Trap Optical Tweezers with Confocal Fluorescence MicroscopeS10OD032158 · OD · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI VAN HOUTEN, BENNETT · 2023 to 2023
$552k
Base Excision Repair: Mechanisms of DNA Damage Access and Repair in ChromatinF32GM140718 · NIGMS · UNIVERSITY OF KANSAS MEDICAL CENTER · PI WEAVER, TYLER MATHEW · 2021 to 2022
$134k
DNA repair pathway coordination during damage processingF32ES034982 · NIEHS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI SCHAICH, MATTHEW ALLEN · 2023 to 2025
$131k
Dynamics of oxidative DNA demethylation from duplex DNA to chromatinK99ES036599 · NIEHS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI SCHAICH, MATTHEW ALLEN · 2025 to 2025
$127k
NCI NIH HHS P01 CA092584NCI NIH HHS P30 CA047904NCI NIH HHS P30 CA118100NIEHS NIH HHS F32 ES034982NIEHS NIH HHS K99 ES036599NIEHS NIH HHS R01 ES012512NIEHS NIH HHS R35 ES031638NIGMS NIH HHS F32 GM140718NIGMS NIH HHS R35 GM128562NIH HHS S10 OD032158U.S. Department of Health & Human Services | NIH | National Institute of Environmental Health Sciences (NIEHS) 2P30CA047904U.S. Department of Health & Human Services | NIH | National Institute of Environmental Health Sciences (NIEHS) S10OD032158
6 · The paper itself
Abstract
Ligation of DNA single strand breaks is critical for maintaining genome integrity during DNA replication and repair. DNA Ligase III (LIG3α) forms an important complex with X-ray cross complementing protein 1 (XRCC1) during single strand break and base excision repair. We utilize a real time single molecule approach to quantify DNA binding kinetics of HaloTag-LIG3α and XRCC1-YFP from nuclear extracts on long DNA substrates containing nicks, nucleosomes or nicks embedded in nucleosomes. LIG3α displays higher affinity for nicks than XRCC1 with the LIG3α catalytic core and N-terminal zinc finger (ZnF) competing for nick engagement. Surprisingly, compared to single strand breaks in naked DNA, LIG3α binds even more avidly to an undamaged nucleosome reconstituted on the 601-sequence, with binding dependent on two arginine residues in the N-terminal ZnF. These studies reveal insights into nick detection and identify an arginine anchor mechanism for LIG3α engagement with nucleosomes.
Indexed as
ArginineDNA Ligase ATPNucleosomesZinc FingersAnimalsDNADNA Breaks, Single-StrandedDNA RepairHumansKineticsPoly-ADP-Ribose Binding ProteinsProtein BindingXenopus ProteinsX-ray Repair Cross Complementing Protein 1ArginineDNADNA Ligase ATPDNA ligase III alpha protein, XenopusLIG3 protein, humanNucleosomesPoly-ADP-Ribose Binding ProteinsXenopus ProteinsX-ray Repair Cross Complementing Protein 1XRCC1 protein, human
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.
The zinc finger of DNA ligase 3α binds to nucleosomes via an arginine anchor. · full record | OpenQuestion