Evidence map›Paper›PMID 41270925›Full record

ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2025

A Systemic Approach to Chest Radiographic Assessment in Mycobacterium tuberculosis-Infected Cynomolgus Macaques (Macaca fascicularis).

Taratorn Kemthong, Mutchamon Kaewparuehaschai, Kirana Noradechanon, Keerati Kongrod, Suthirote Meesawat, Saradee Warit, Prapaporn Srilohasin, Angkana Chaiprasert, Suchinda Malaivijitnond

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Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Taratorn Kemthong1Biological Sciences Program, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
Mutchamon Kaewparuehaschai2Wildlife Conservation Office, Department of National Parks, Wildlife and Plant Conservation, Bangkok, Thailand.
Kirana Noradechanon2Wildlife Conservation Office, Department of National Parks, Wildlife and Plant Conservation, Bangkok, Thailand.
Keerati Kongrod3National Primate Research Center of Thailand, Chulalongkorn University, Saraburi, Thailand.
Suthirote Meesawat3National Primate Research Center of Thailand, Chulalongkorn University, Saraburi, Thailand.
Saradee Warit4National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand.
Prapaporn Srilohasin5Office for Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; and.
Angkana Chaiprasert5Office for Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; and.
Suchinda Malaivijitnond1Biological Sciences Program, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to establish a comprehensive and accurate numerical chest X-ray radiograph (CXR) scoring system in cynomolgus macaques by using image intensity values from healthy, tuberculosis (TB)-free animals as references. The CXRs were obtained in both dorsoventral and lateral postures from 90 macaques and viewed by the RadiAnt DICOM Viewer software version 2023.1. The mean and maximum intensity values were analyzed and showed significant differences between sex (male and female) and age class (juvenile and subadult/adult), varying based on body sizes. The cutoff values were, therefore, set separately and were tested for accuracy in detecting TB status in 18 naturally Mycobacterium tuberculosis-infected macaques, which were assessed for active tuberculosis infection (ATBI) using Xpert MTB/RIF Ultra at least once during a 12-month follow-up. Only the cutoff values of maximum lateral image intensity (MLIs) correctly identified TB infection in 100% of cases. Thus, the MLIs were selected to follow up on the development of TB lesions in those 18 Mycobacterium tuberculosis-infected macaques. The lateral digital radiograph was divided further into 9 areas, and the MLIs can predict the progression of TB lesions, which were most likely located in the dorsal part of the cranial lung lobe between thoracic vertebrae 1 (T1) to T4. Finally, the CXR results of another group of 8 Mycobacterium tuberculosis-exposed macaques, whose TB status was either uninfected, latent, or ATBI, were compared between a blind test by an expert radiologist and our established CXR scoring system. The blind test results showed 62.5% (5/8) agreement with our scoring system. This suggests that the CXR-MLI scoring system can be used as a supplementary tool for TB diagnosis in cynomolgus macaques.

Indexed as

ATBI, active tuberculosis infectionCXR, chest X-ray radiographDNP, Department of the National Parks, Wildlife and Plant Conservation of ThailandIGRA, interferon-gamma release assaysKBK, Krabok-Koo Wildlife Breeding CenterLTBI, latent tuberculosis infectionMLIs, maximum lateral image intensitiesNHP, nonhuman primateNPRCT-CU, National Primate Research Center of Thailand-Chulalongkorn UniversityTB, tuberculosisTST, intradermal tuberculin skin test

Identifiers

PMID41270925
PMCPMC12694260

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.