Evidence map›Paper›PMID 41270736›Full record

ArticleNeuron2025

A transcriptomic atlas of astrocyte heterogeneity across space and time in mouse and marmoset.

Margaret E Schroeder, Dana M McCormack, Lukas R Metzner, Jinyoung Kang, Katelyn X Li, Eunah Yu, Lisa Melamed, Kirsten M Levandowski, Heather Zaniewski, Qiangge Zhang and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuron, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
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  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Review
  11. Cross-species consensus atlas of the primate basal ganglia.bioRxiv : the preprint server for biology · 2025
    Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Margaret E SchroederMcGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA.
Dana M McCormackMcGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA.
Lukas R MetznerMcGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA.
Jinyoung KangMcGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA.
Katelyn X LiMcGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA.
Eunah YuMcGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA.
Lisa MelamedMcGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA.
Kirsten M LevandowskiMcGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; The Broad Institute of MIT and Harvard, Cambridge, MA 02139, USA.
Heather ZaniewskiMcGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA.
Qiangge ZhangMcGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; The Broad Institute of MIT and Harvard, Cambridge, MA 02139, USA.
Edward S BoydenMcGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA; Yang Tan Collective, MIT, Cambridge, MA 02139, USA; Center for Neurobiological Engineering and K. Lisa Yang Center for Bionics, MIT, Cambridge, MA 02139, USA; Department of Biological Engineering, MIT, Cambridge, MA 02139, USA; Koch Institute, MIT, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Cambridge, MA 02139, USA; Media Arts and Sciences, MIT, Cambridge, MA 02139, USA.
Fenna M KrienenPrinceton Neuroscience Institute, Princeton University, Princeton, NJ 08544, USA.
Guoping FengMcGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA; Yang Tan Collective, MIT, Cambridge, MA 02139, USA; The Broad Institute of MIT and Harvard, Cambridge, MA 02139, USA. Electronic address: fengg@mit.edu.

Funding

Functionally guided adult whole brain cell atlas in human and NHPUM1MH130981 · NIMH · ALLEN INSTITUTE · PI Ed Lein, Hongkui Zeng · 2022 to 2026
$91.9M
BRAIN CONNECTS: Comprehensive Regional Projection Map of Marmoset with Single Axon and Cell Type ResolutionUM1NS132173 · NINDS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI Guoping Feng, PARTHA Pratim MITRA · 2023 to 2026
$11.6M
A Molecular and Cellular Atlas of the Marmoset BrainU01MH114819 · NIMH · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI FENG, GUOPING · 2017 to 2021
$6.9M
Expansion MicroscopyR01EB024261 · NIBIB · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2017 to 2025
$4.6M
NIBIB NIH HHS R01 EB024261NIMH NIH HHS U01 MH114819NIMH NIH HHS UM1 MH130981NINDS NIH HHS UM1 NS132173
6 · The paper itself

Abstract

How astrocyte regionalization unfolds over development is not fully understood. We used single-nucleus RNA sequencing to characterize the molecular diversity of brain cells across six developmental stages and four brain regions in the mouse and marmoset brain. Our analysis revealed striking regional heterogeneity among astrocytes, particularly between telencephalic and diencephalic regions in both species. Most of the region patterning was private to astrocytes and not shared with neurons or other glial types. Though astrocytes were already regionally patterned in late embryonic stages, this region-specific astrocyte gene expression signature changed significantly over postnatal development, and its composition suggests that regional astrocytes further specialize postnatally to support their local neuronal circuits. Across mouse and marmoset, we found hundreds of species-differentially expressed genes and divergence in the expression of astrocytic region- and age-differentially expressed genes. Finally, we used expansion microscopy to show that astrocyte morphology is also regionally specialized.

Indexed as

AstrocytesBrainTranscriptomeAnimalsCallithrixGene Expression Regulation, DevelopmentalMiceMice, Inbred C57BLastrocyteexpansion microscopyheterogeneitymarmosetmouseneurodevelopmentsnRNA-seqtranscriptomics

Identifiers

PMID41270736
PMCPMC12989161

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.