ArticleBioresources and bioprocessing2025
Thermophilic fermentation enhanced by eggshell-derived hydroxyapatite for sustainable hydrogen production.
Article in Bioresources and bioprocessing, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
This study presents the eco-friendly synthesis of Hydroxyapatite (HAP) using chicken eggshell biowaste as a sustainable calcium source to enhance biohydrogen production. The stoichiometric ratio of Ca/P was determined to be 1.67 by evaluating pH, temperature, and calcination time, revealing that pH 10.5, temperature 950ᵒC, and calcination time of 2 h were optimal. Structural and morphological characterization confirmed the successful formation of crystalline HAP with 78% crystallinity (XRD), accurate Ca/P ratio (EDS), and phosphate group presence (FTIR). The synthesized HAP was applied as a bio-additive in thermophilic dark fermentation using a Thermoanaerobacterium-enriched sludge. The optimal HAP dosage (560 mg/L) yielded the highest hydrogen yield of 1.085 mol H
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