ArticleMethods in molecular biology (Clifton, N.J.)2026
Assessing Mitochondrial Respiration of In Vitro Models for the Study of Immune-Related Diseases Using a Seahorse Analyzer.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Energy metabolism, particularly mitochondrial function, has emerged as a key regulator of the immune response and plays a significant role in the pathophysiology of inflammatory diseases. Studying mitochondrial parameters is therefore crucial in understanding these pathologies. This protocol outlines the evaluation of mitochondrial respiration parameters using a Seahorse Analyzer. With the growing use of innovative models that better mimic human physiology, such as cell cultures in inserts, this chapter provides a detailed guide to Seahorse analysis for this specific type of culture.
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