ArticleMethods in molecular biology (Clifton, N.J.)2026
Neutrophil Transmigration Analyses for Multiplexed Drug Screening and In Vivo Like Application Testing Using an Organ on a Chip Model.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Neutrophils are the most abundant type of innate immune cell in humans and make up for 40-60% of leukocytes in humans. As a part of the innate immunity and due to their high frequency, they form the first line of defense against harmful invaders. While neutrophils are crucial for combating infections, dysregulated neutrophil activity is a hallmark of chronic inflammation and autoimmune disorders. Here, we describe two methods to evaluate neutrophil migration, an in vitro transwell migration assay and an ex vivo organ-on-chip model, which allows quantification of the migration of neutrophils under flow and through human umbilical vein endothelial cells to best mimic conditions found in the human body.
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