Evidence map›Paper›PMID 41269547›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Culturing Murine Bone Marrow-Derived Macrophages.

Becky Lin, Giraldina Trevejo-Nuñez

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Becky LinDivision of Infectious Diseases, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Giraldina Trevejo-NuñezDivision of Infectious Diseases, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. giraldina.trevejo@pitt.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bone marrow-derived macrophages (BMDMs) mimic several properties of macrophages, such as phagocytosis and antigen processing and presentation, and may be used to study and characterize macrophage biology in response to specific stimuli. BMDMs are generated through isolation of cells from murine femur and tibia and cultured with L929-conditioned media, which contain factors essential for differentiation into macrophages. This protocol details the steps of retrieving bone marrow from mice, collecting L929-condition media, and culturing bone marrow cells to differentiate into macrophages.

Indexed as

Bone Marrow CellsCell Culture TechniquesMacrophagesAnimalsCell DifferentiationCells, CulturedCulture Media, ConditionedMiceCulture Media, ConditionedBone marrow-derived macrophages (BMDMs)In vitro cell cultureMacrophage colony-stimulating factor (M-CSF)Monocyte differentiationMurine bone marrow

Identifiers

PMID41269547

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.