Evidence map›Paper›PMID 41269544›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Identification and Characterization of Human Autoreactive T Cells for Autoimmune Research.

Lenka Súkeníková, Daniela Latorre

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Lenka SúkeníkováInstitute of Microbiology, ETH Zurich, Zurich, Switzerland.
Daniela LatorreInstitute of Microbiology, ETH Zurich, Zurich, Switzerland. latorre.daniela@hsr.it.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

T cells directed against self-antigens play a pivotal role in autoimmunity, yet their comprehensive characterization in human autoimmune disorders remains challenging. In this chapter, we present methods to isolate and profile autoreactive T cells from human biological samples, specifically peripheral blood, cerebrospinal fluid, and tissue biopsies. The workflow combines in vitro T cell screening, isolation of autoreactive T cells, and single T cell clone generation, followed by in depth analysis of T cell receptor (TCR) repertoire, HLA restriction, cytokine profile, and targeted self-epitope. These protocols aim to facilitate research of human autoimmune diseases by providing powerful tools for systematic analysis of autoreactive T cells in human biological samples.

Indexed as

Autoimmune DiseasesAutoimmunityT-LymphocytesAutoantigensCell SeparationCytokinesFlow CytometryHumansReceptors, Antigen, T-CellSingle-Cell AnalysisAutoantigensCytokinesReceptors, Antigen, T-CellAutoimmunityAutoreactive T cellsEpitope specificitySingle T cell cloneT cell receptor (TCR)

Identifiers

PMID41269544

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.