ArticleBiomedical microdevices2025
Automation of electrothermal cell sheet manipulator for seamless tissue assembly and handling.
Article in Biomedical microdevices, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Engineering Multiscale Vasculature: Biological Principles, Design Constraints, and Advanced Biofabrication Strategies for Functional Vascular Networks.Biomimetics (Basel, Switzerland) · 2026Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The manipulation of fragile biological tissues such as engineered cell sheets remains a major challenge for regenerative medicine and tissue engineering. Manual handling with tools like tweezers often induces wrinkling or tearing, compromising tissue integrity. Here, we present an automated cell sheet manipulator that integrates a thermoresponsive microchanneled poly(N-isopropylacrylamide) (PNIPAAm) hydrogel with an embedded microheater, mounted on a programmable three-axis motorized stage. Upon localized heating and cooling, the hydrogel undergoes rapid, reversible volumetric transitions that enable suction-based gripping and release of cell sheets within a few seconds. The custom LabVIEW interface synchronizes stage movement and thermal cycling, allowing reproducible, hands-free operation. A compliance-based Z-axis apparatus ensured uniform low-magnitude contact forces, preventing mechanical damage during transfer. Using this system, human iPSC-derived neural sheets were reliably transferred onto human brain microvascular endothelial cell (hBMEC) monolayers. Compared to manual transfer, the automated manipulator preserved cell sheet flatness and minimized micro-wrinkling, resulting in safe retention of intercellular architecture and structural integrity. This work demonstrates a robust, user-friendly platform for automated and gentle handling of delicate biological sheets. By enabling the precise stacking of engineered tissues while preserving their morphology, this system provides a promising tool for advanced biofabrication workflows, supporting defect-free 3D tissue assembly and implantation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.