ArticleBMC genomics2025
Haplotype-resolved genome assembly of 'Manhattan' perennial ryegrass (Lolium perenne L.) and characterization of drought responsive late embryogenesis abundant genes.
Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- A haplotype-resolved genome assembly of hexaploid Kentucky-31 tall fescue (Lolium arundinaceum).G3 (Bethesda, Md.) · 2026Article
- Haplotype-resolved chromosome-level genome assembly of creeping bentgrass, Agrostis stolonifera.Scientific data · 2026Article
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5 authors.
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Abstract
backgroundPerennial ryegrass is a premier forage and turf grass, a genomic model organism for cool-season perennial grasses, but has relatively poor persistence under drought stress. The cultivar 'Manhattan' is an heirloom turf-type cultivar, and ancestral to many current turf cultivars and breeding lines in the USA. To improve turf-type perennial ryegrass genome resources, we assembled and compared two haplotypes of 'Manhattan' and extracted late embryogenesis abundant (LEA) gene families.
resultsBoth haplotypes resolved into 2.3 Gb genome assemblies of 7 chromosomes each, with Gypsy-like retrotransposon concentrations in putative centromere regions. Repeat content was 83%, and the two haplotypes were syntenic with each other as well as published forage-type perennial ryegrass genomes. Annotations resulted in 43,000 genes for each haplotype with over 95% complete, 89% as single copy genes, and 86% with functional annotation. Seventy-two LEA genes were identified in haplotype-1 and fitted into 8 Pfam-based families, with 46 exhibiting expression evidence in vegetative tissues and 39 showing differential expression upon drought stress.
conclusionsBroad synteny but high heterozygosity characterized the 'Manhattan' perennial ryegrass genome haplotypes. Repeat content, including long terminal repeat genes with annotation support, were high and indicative of cool-season Poaceae grasses. The identification of LEA genes differentially expressed upon drought stress provide candidate genes for further drought tolerance studies.
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