Evidence map›Paper›PMID 41266980›Full record

ArticleBMC plant biology2025

Deciphering the genetic basis of chlorophyll fluorescence parameters and vegetation indices under heat stress in blueberry.

Krishnanand P Kulkarni, Jodi Callwood, Kalpalatha Melmaiee, Jennifer Johnson-Cicalese, Nicholi Vorsa, Umesh K Reddy, Purushothaman Natarajan, Sathya Elavarthi, Massimo Iorizzo

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Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Krishnanand P KulkarniDepartment of Agriculture and Natural Resources, Delaware State University, Dover, DE, 19901, USA.
Jodi CallwoodDepartment of Agriculture and Natural Resources, Delaware State University, Dover, DE, 19901, USA.
Kalpalatha MelmaieeDepartment of Agriculture and Natural Resources, Delaware State University, Dover, DE, 19901, USA. kmelmaiee@desu.edu.
Jennifer Johnson-CicaleseDepartment of Plant Biology, Rutgers University, New Brunswick, NJ, 08901, USA.
Nicholi VorsaDepartment of Plant Biology, Rutgers University, New Brunswick, NJ, 08901, USA.
Umesh K ReddyDepartment of Biology, West Virginia State University, Institute, WV, 25112, USA.
Purushothaman NatarajanDepartment of Agriculture, Food, and Resource Sciences, University of Maryland Eastern Shore, Princess Anne, MD, 21853, USA.
Sathya ElavarthiDepartment of Agriculture and Natural Resources, Delaware State University, Dover, DE, 19901, USA.
Massimo IorizzoDepartment of Horticultural Science and Plants, for Human Health Institute, NC State University, Kannapolis, NC, 28081, USA.

Funding

National Institute of Food and Agriculture 2018-38821-27744, 2018-67014-27622, and 2023-38821-39921
6 · The paper itself

Abstract

backgroundBlueberry (Vaccinium section Cyanococcus) is a commercially important fruit crop. Its cultivation is challenging due to specific climate and soil requirements, with even minor temperature fluctuations potentially affecting growth and fruit yield. Heat stress impairs growth and photosynthesis by causing damage to the photosystem II (PSII) complex. Hence, identifying genomic regions influencing the maximum quantum efficiency of PSII and the overall photosynthetic capacity of plants under heat stress is crucial.

resultsIn this study, we exposed 266 interspecific cross derivatives of blueberry plants to heat stress in controlled conditions for two consecutive years and evaluated their responses through phenotypic, chlorophyll fluorescence, and vegetation indices measurements. We observed continuous and significant variation for all the measured traits, indicating their quantitative nature. Genome-wide association studies (GWAS) using 126,816 single-nucleotide polymorphisms revealed several candidate genes encoding molecular chaperones, serine-threonine kinases, and DEAD-box proteins, which play critical roles in heat stress responses in plants. Additionally, we identified 10,393 differentially expressed genes (DEGs) from the transcriptomic analysis of V. corymbosum and V. darrowii plants subjected to 6 and 9 hours of heat stress. RNA-Seq data were further confirmed by quantitative real-time PCR analysis of randomly selected genes. Functional characterization through Gene Ontology and pathway analysis of 325 genes commonly expressed in all four comparison groups indicated that DEGs were mainly enriched in protein processing in the endoplasmic reticulum. Through GWAS and transcriptomic analysis, we uncovered 23 genes on chromosomes 1, 2, 3, 4, 5, 7, 8, 9, 10, and 12 associated with chlorophyll fluorescence parameters and vegetation indices under heat stress.

conclusionsThis study provides a deeper understanding of the genetic basis of chlorophyll fluorescence under heat stress in blueberries, offering valuable information for breeding strategies to develop blueberry cultivars with enhanced photosynthetic efficiency.

Indexed as

Blueberry PlantsChlorophyllHeat-Shock ResponseFluorescenceGenome-Wide Association StudyPhotosynthesisPolymorphism, Single NucleotideChlorophyllBlueberryChlorophyll fluorescenceGWASHeat stressSingle nucleotide polymorphismTranscriptome analysis

Identifiers

PMID41266980
PMCPMC12632021

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.