Evidence map›Paper›PMID 41266738›Full record

ArticleScientific reports2025

Ethylenediaminetetraacetic acid conjugated with polyethylene glycol is a safe chelator for colonic epithelial denudation and intestinal epithelial replacement therapy.

Yuka Matsumoto, Yuta Murakami, Kazuto Suda, Mirei Takahasi, Kenichiro Nakai, Nozomi Ohta, Shihei Motofuji, Go Miyano, Hiroyuki Koga, Nobutaka Hattori and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yuka MatsumotoDepartment of Pediatric General and Urogenital Surgery, Juntendo University School of Medicin, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan. y.matsumoto.sg@juntendo.ac.jp.
Yuta MurakamiSanyo Chemical Industries, Ltd, 11-1, Ikkyo Nomoto-cho, Higashiyama-ku, 6050995, Kyoto, Kyoto-shi, Japan.
Kazuto SudaDepartment of Pediatric General and Urogenital Surgery, Juntendo University School of Medicin, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
Mirei TakahasiDepartment of Pediatric General and Urogenital Surgery, Juntendo University School of Medicin, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
Kenichiro NakaiSanyo Chemical Industries, Ltd, 11-1, Ikkyo Nomoto-cho, Higashiyama-ku, 6050995, Kyoto, Kyoto-shi, Japan.
Nozomi OhtaSanyo Chemical Industries, Ltd, 11-1, Ikkyo Nomoto-cho, Higashiyama-ku, 6050995, Kyoto, Kyoto-shi, Japan.
Shihei MotofujiSanyo Chemical Industries, Ltd, 11-1, Ikkyo Nomoto-cho, Higashiyama-ku, 6050995, Kyoto, Kyoto-shi, Japan.
Go MiyanoDepartment of Pediatric General and Urogenital Surgery, Juntendo University School of Medicin, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
Hiroyuki KogaDepartment of Pediatric General and Urogenital Surgery, Juntendo University School of Medicin, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
Nobutaka HattoriDepartment of Neurology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
Kiichiro TsuchiyaDepartment of Gastroenterology, Institute of Medicine, University of Tsukuba, 1-1, Amakubo 2-chome, Tsukuba City, 305-0005, Ibaraki Prefecture, Japan.
Atsuyuki YamatakaDepartment of Pediatric General and Urogenital Surgery, Juntendo University School of Medicin, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
Tetsuya NakamuraDepartment of Research and Development for Organoids, Juntendo University Graduate School, 2-1-1, Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan. t.nakamura.sv@juntendo.ac.jp.

Funding

Japan Agency for Medical Research and Development 22hma322002h0001Japan Society for the Promotion of Science 19K07500Japan Society for the Promotion of Science 22H03138Japan Society for the Promotion of Science 23K08038
6 · The paper itself

Abstract

Colonic epithelia can be replaced with small intestinal (SI) epithelia through de-epithelialization of the colon using ethylenediaminetetraacetic acid (EDTA), followed by SI organoid transplantation, to treat short bowel syndrome and intestinal failure. However, the low molecular weight (MW) of EDTA results in hypocalcemia, consequently hindering the clinical application of this bivalent cation chelator. Therefore, we aimed to synthesize a non-absorbable chelator for potential application in regenerative medicine. We conjugated polyethylene glycols (PEGs) of different MWs to EDTA to synthesize EDTA-PEGs with higher MWs. NMR and LC-TOF/MS analyses demonstrated the stability and chelating ability of EDTA-PEGs. Moreover, EDTA-PEGs mitigated hypocalcemia in mice. This effect was more pronounced in EDTA-PEGs with a higher MW than in EDTA. Furthermore, EDTA-PEGs de-epithelialized a targeted region of the mouse colon, replacing it with SI organoids to preserve SI features. This study provides a basis for the development of safe regenerative medicine utilizing EDTA-PEG.

Indexed as

Chelating AgentsColonEdetic AcidIntestinal MucosaPolyethylene GlycolsAnimalsHumansMiceOrganoidsChelating AgentsEdetic AcidPolyethylene GlycolsChelating agentsIntestinal organoidsRegenerative medicineTransplantation

Identifiers

PMID41266738
PMCPMC12635222

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.