Evidence map›Paper›PMID 41266667›Full record

ArticleScientific reports2025

A multi-omics approach exploring the gut-liver axis following combined radiation exposure and burn injury in a Sinclair minipig model.

Timothy S Horseman, Babita Parajuli, Veda Murthy, Gregory P Holmes-Hampton, Gauthaman Sukumar, Clifton L Dalgard, Joseph A Anderson, David M Burmeister

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Timothy S HorsemanSchool of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD, 20814, USA.
Babita ParajuliSchool of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD, 20814, USA.
Veda MurthyArmed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, Bethesda, MD, 20814, USA.
Gregory P Holmes-HamptonArmed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, Bethesda, MD, 20814, USA.
Gauthaman SukumarThe American Genome Center, Center for Military Precision Health, Uniformed Services University of the Health Sciences, Bethesda, MD, 20814, USA.
Clifton L DalgardThe American Genome Center, Center for Military Precision Health, Uniformed Services University of the Health Sciences, Bethesda, MD, 20814, USA.
Joseph A AndersonComparative Pathology Division, Department of Laboratory Animal Resources, Uniformed Services University of the Health Sciences, Bethesda, MD, 20814, USA.
David M BurmeisterSchool of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD, 20814, USA. David.burmeister@usuhs.edu.

Funding

United States Army Medical Research and Development Command's Combat Casualty Care Program RHE-24-01
6 · The paper itself

Abstract

While radiation and burn injury have distinct local and systemic effects, they both negatively impact intestinal permeability/function. In a nuclear attack, combined thermal burns and radiation exposure (e.g., combined injury (CI)) would be a dominant injury pattern. Despite this, how burns affect gastrointestinal acute radiation syndrome, and vice versa, has been largely unstudied. Next-generation sequencing has revealed a strong bidirectional link between the liver and gut. Here, we used a porcine model to evaluate the impact of burn, radiation, and CI on the gut microbiota and liver transcriptomics to determine if this link exists in these injury patterns. Sinclair minipigs were randomly divided into three groups: burn (n = 8), hemibody radiation (n = 7), and CI (n = 8). Animals were monitored for 14 days with longitudinal rectal swab and blood collection. CI increased weight loss, diarrhea, inappetence and lethargy compared to either injury pattern alone. Jejunum histology revealed increased mucosal apoptosis in burn and CI groups compared to radiation. CI led to elevated levels of NLRP3 and IL1β in the jejunum. Intestinal barrier disruption was indicated by decreasing circulating l-citrulline in CI which correlated inversely with intestinal-Fatty Acid Binding Protein. Bacteremia was elevated post-burn on day 1 and again from day 7-14 in burn and CI groups. Microbiome analysis showed phylogenetic shifts and differential abundance across groups with CI animals exhibiting distinct microbial signatures linked to intestinal dysfunction and liver injury. Liver RNA-seq revealed group-specific gene expression changes, with CI-altered pathways implicating immune responses and lipid metabolism, which were correlated with gut microbiota such as Bacteroidaceae and Lachnospiraceae. Taken together, we present a first-of-its kind large animal model of radiation combined injury to highlight the interplay between gut-liver axis disruptions and systemic injury responses.

Indexed as

BurnsGastrointestinal MicrobiomeLiverRadiation ExposureRadiation Injuries, ExperimentalAnimalsDisease Models, AnimalMultiomicsSwineSwine, MiniatureTranscriptome

Identifiers

PMID41266667
PMCPMC12635091

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.