Evidence map›Paper›PMID 41266540›Full record

ArticleScientific reports2025

Extracellular mitochondrial DNA activates complement and is associated with complement activation in patients with out-of-hospital cardiac arrest.

Eline de Boer, Anne-Lise Strandmoe, Marina Sokolova, Trond M Michelsen, Huy Q Quach, Viktoriia Chaban, Espen R Nakstad, Geir Ø Andersen, May-Kristin S Torp, Kåre-Olav Stensløkken and 2 more

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Eline de BoerInstitute of Clinical Medicine, University of Oslo, Oslo, Norway.
Anne-Lise StrandmoeInstitute of Clinical Medicine, University of Oslo, Oslo, Norway.
Marina SokolovaInstitute of Clinical Medicine, University of Oslo, Oslo, Norway.
Trond M MichelsenInstitute of Clinical Medicine, University of Oslo, Oslo, Norway.
Huy Q QuachInstitute of Clinical Medicine, University of Oslo, Oslo, Norway.
Viktoriia ChabanInstitute of Clinical Medicine, University of Oslo, Oslo, Norway.
Espen R NakstadDepartment of Acute Medicine, Oslo University Hospital, Oslo, Norway.
Geir Ø AndersenDepartment of Cardiology, Oslo University Hospital, Oslo, Norway.
May-Kristin S TorpInstitute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
Kåre-Olav StensløkkenInstitute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
Tom E MollnesInstitute of Clinical Medicine, University of Oslo, Oslo, Norway.
Søren E PischkeInstitute of Clinical Medicine, University of Oslo, Oslo, Norway. s.e.pischke@medisin.uio.no.ORCID http://orcid.org/0000-0003-2543-3251

Funding

Norges Forskningsråd 274352
6 · The paper itself

Abstract

Synthetic analogues of mitochondrial DNA (mtDNA) have been reported as potent complement system activators. This study investigated the impact of endogenous mtDNA on complement activation. mtDNA and nuclear DNA (nDNA) were extracted from human placental tissue and complement activation was determined using ELISA. When incubated in lepirudin-anticoagulated human blood and plasma, increasing concentrations of mtDNA, but not nDNA, resulted in dose- and time-dependent increases in C3bc, C3bBbP and soluble C5b-9 (sC5b-9). In a clinical context, mtDNA, nDNA, and complement levels of 55 resuscitated out-of-hospital cardiac arrest (OHCA) patients were determined using qPCR and ELISA. C3bc and sC5b-9 correlated significantly with mtDNA and nDNA in OHCA patients. We conclude that mtDNA, but not nDNA, triggers in vitro complement activation, which holds significance in a clinical context in OHCA patients. This novel mode of human complement activation might explain pathophysiological mechanisms of sterile inflammation and could be relevant for innovative therapeutic strategies.

Indexed as

Complement ActivationComplement System ProteinsDNA, MitochondrialOut-of-Hospital Cardiac ArrestAdultAgedComplement Membrane Attack ComplexFemaleHumansMaleMiddle AgedPlacentaPregnancyComplement Membrane Attack ComplexComplement System ProteinsDNA, Mitochondrial

Identifiers

PMID41266540
PMCPMC12635304

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.