Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registry
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Thiago Luiz Alves E SilvaLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA. thiagoluiz.alvesesilva@nih.gov.ORCID http://orcid.org/0000-0002-8520-5405
Sachi KanataniThe W. Harry Feinstone Department of Molecular Microbiology and Immunology and Johns Hopkins Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA.ORCID http://orcid.org/0000-0002-7072-0447
Ana Beatriz Barletta FerreiraLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA.ORCID http://orcid.org/0000-0001-9913-3775
Cindi L SchwartzMicroscopy Unit, Research Technologies Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.ORCID http://orcid.org/0000-0002-0490-6040
Octavio A C TalyuliLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA.
Janet OlivasLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA.
Bianca M NagataInfectious Disease Pathogenesis Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA.
Zarna Rajeshkumar PalaLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA.
Tales PasciniLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA.
Derron A AlvesInfectious Disease Pathogenesis Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA.
Ming ZhaoResearch Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA.ORCID http://orcid.org/0000-0002-5700-2950
Motoshi SuzukiResearch Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA.ORCID http://orcid.org/0000-0002-4346-7677
Lilian P DornerIntegrative Parasitology, Center for Infectious Diseases, University of Heidelberg Medical School, Heidelberg, Germany.ORCID http://orcid.org/0000-0002-8120-7155
Friedrich FrischknechtIntegrative Parasitology, Center for Infectious Diseases, University of Heidelberg Medical School, Heidelberg, Germany.ORCID http://orcid.org/0000-0002-8332-6668
Isabelle CoppensThe W. Harry Feinstone Department of Molecular Microbiology and Immunology and Johns Hopkins Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA.ORCID http://orcid.org/0000-0001-5549-2362
Carolina Barillas-MuryLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA.ORCID http://orcid.org/0000-0002-4039-6199
Jose M C RibeiroLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA.
Photini SinnisThe W. Harry Feinstone Department of Molecular Microbiology and Immunology and Johns Hopkins Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA.ORCID http://orcid.org/0000-0003-2954-7547
Joel Vega-RodriguezLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA. joel.vega-rodriguez@nih.gov.ORCID http://orcid.org/0000-0002-9576-0058
Funding
Vector-Borne Diseases: Biology Of Vector Host RelationshipZ01AI000810 · NIAID · NIAID EXTRAMURAL ACTIVITIES · PI RIBEIRO, JOSE M · 1997 to 2008
$3.5M
The Skin Phase of Malaria Infection (Sharon Patray Diversity Supplement)R01AI132359 · NIAID · JOHNS HOPKINS UNIVERSITY · PI SINNIS, PHOTINI · 2017 to 2021
$2.7M
Mosquito Midgut Immune Responses &Malaria TransmissionZ01AI000947 · NIAID · NIAID EXTRAMURAL ACTIVITIES · PI BARILLAS-MURY, CAROLINA · 2004 to 2008
$1.8M
Image-based quantification of Plasmodium sporozoites in a single oocyst using deep learning-based segmentation and 3D reconstructionR03AI180804 · NIAID · JOHNS HOPKINS UNIVERSITY · PI KANATANI, SACHIE, SINNIS, PHOTINI · 2024 to 2025
$161k
Department of Education and Training | Australian Research Council (ARC) DP250102307Deutsche Forschungsgemeinschaft (German Research Foundation) FR2140/12-2Division of Intramural Research, National Institute of Allergy and Infectious Diseases (Division of Intramural Research of the NIAID) Z01 AI000810-21Division of Intramural Research, National Institute of Allergy and Infectious Diseases (Division of Intramural Research of the NIAID) Z01AI000947Intramural NIH HHS Z01 AI000810Intramural NIH HHS Z01 AI000947NIAID NIH HHS R01 AI132359NIAID NIH HHS R03 AI180804U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (NIAID) R01AI132359
6 · The paper itself
Abstract
Plasmodium sporozoites, the infective stage of malaria, must invade the mosquito salivary glands (SGs) before being transmitted to a vertebrate host. However, the physiological and biochemical effects of this invasion remain largely unexplored. We examined the impact of Plasmodium infection on Anopheles gambiae salivary glands using high-resolution proteomics, gene expression, and morphological analysis. The data reveal differential expression of various proteins, including the enrichment of hemolymph-derived humoral proteins in infected salivary glands. These proteins diffuse into the SGs due to structural damage caused by the sporozoites during invasion, while saliva proteins diffuse out into the circulation. Moreover, proteomic analysis of saliva from P. berghei- or P. falciparum-infected mosquitoes revealed changes in composition, with a pronounced reduction of immune proteins relative to uninfected mosquitoes. This reduction is likely due to the association of these proteins with the surface of sporozoites and/or changes in the saliva's physical properties within the invaded salivary secretory cavities. The saliva protein profiles from mosquitoes infected with both Plasmodium species are remarkably similar, suggesting a conserved interaction between sporozoites and salivary glands. Our results provide a foundation for understanding the molecular interactions between Plasmodium sporozoites and mosquito salivary glands.
Indexed as
AnophelesHemolymphInsect ProteinsPlasmodium falciparumSalivaSalivary GlandsAnimalsFemaleHost-Parasite InteractionsMalariaMosquito VectorsPlasmodium bergheiProteomeProteomicsSalivary Proteins and PeptidesSporozoitesInsect ProteinsProteomeSalivary Proteins and Peptides
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.
High-resolution proteomics unveils salivary gland disruption and saliva-hemolymph protein exchange in Plasmodium-infected mosquitoes. · full record | OpenQuestion