Evidence map›Paper›PMID 41266339›Full record

ArticleNPJ biofilms and microbiomes2025

Novel antimicrobial peptides and peptide-microbiome crosstalk in Appalachian salamander skin.

Carly R Muletz-Wolz, Julian Urrutia-Carter, Owen Osborne, Steve Kutos, Jose Meneses Montano, Joseph D Madison, Brian Gratwicke, Ratanachat Racharaks, Norma E Roncal, Randall R Jimenez and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in NPJ biofilms and microbiomes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Advancing the study of microbial symbionts of amphibians and reptiles.Frontiers in amphibian and reptile science · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Carly R Muletz-WolzCenter for Conservation Genomics, Smithsonian's National Zoo & Conservation Biology Institute, Washington, DC, USA. muletzc@si.edu.
Julian Urrutia-CarterCenter for Conservation Genomics, Smithsonian's National Zoo & Conservation Biology Institute, Washington, DC, USA.
Owen OsborneDepartment of Biological Sciences, Bangor University, Bangor, UK.
Steve KutosCenter for Conservation Genomics, Smithsonian's National Zoo & Conservation Biology Institute, Washington, DC, USA.
Jose Meneses MontanoCenter for Conservation Genomics, Smithsonian's National Zoo & Conservation Biology Institute, Washington, DC, USA.
Joseph D MadisonCenter for Conservation Genomics, Smithsonian's National Zoo & Conservation Biology Institute, Washington, DC, USA.
Brian GratwickeCenter for Species Survival, Smithsonian's National Zoo & Conservation Biology Institute, Front Royal, VA, USA.
Ratanachat RacharaksWalter Reed Army Institute of Research, Bethesda, MD, USA.
Norma E RoncalWalter Reed Army Institute of Research, Bethesda, MD, USA.
Randall R JimenezCenter for Conservation Genomics, Smithsonian's National Zoo & Conservation Biology Institute, Washington, DC, USA.
Amy EllisonDepartment of Biological Sciences, Bangor University, Bangor, UK.
Timothy P ClelandSmithsonian Museum Conservation Institute, Suitland, MD, USA.

Funding

Biotechnology and Biological Sciences Research Council BB/W013517/1National Science Foundation IOS-2131060Walter Reed Army Institute of Research MI210200
6 · The paper itself

Abstract

Using multi-omics tools, we discovered new antimicrobial peptides (AMPs) and examined AMP-microbial interactions in three Appalachian salamander species (Plethodon cinereus, Eurycea bislineata and Notophthalmus viridescens). We conducted skin transcriptomics (n = 13) and proteomics (n = 91) to identify 200+ candidate AMPs. With candidate AMPs, we identified correlations with skin microbiomes and synthesized 20 peptides to challenge against pathogens of amphibians (Batrachochytrium dendrobatidis: Bd) and humans (ESKAPEE). Using transcriptomics, candidate AMPs were detected in all individuals with Cathelidicins being most common. Using proteomics, AMPs were found in 34% of individuals (31/91)-predominately E. bislineata-with Kinin-like peptides being most common. Candidate AMP composition generally predicted skin bacterial composition, suggesting that AMPs influence host-microbial symbioses. Crude and synthesized peptides showed limited activity against Bd. Two synthesized Cathelicidins (Pcin-CATH3 and Pcin-CATH5) inhibited human pathogens, Acinetobacter baumannii, Pseudomonas aeruginosa and Escherichia coli. Our findings inform the potential usage of AMPs in conservation and translational applications.

Indexed as

Antimicrobial Cationic PeptidesAntimicrobial PeptidesCaudataMicrobiotaSkinAnimalsBacteriaBatrachochytriumGene Expression ProfilingHumansProteomicsAntimicrobial Cationic PeptidesAntimicrobial Peptides

Identifiers

PMID41266339
PMCPMC12635268

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.