Evidence map›Paper›PMID 41265809›Full record

ArticleAnalytical biochemistry2026

One-step purification of a bioactive PAK1-derived peptide.

Djamali Muhoza, Emily P Esquivel, Stacy R Hunter, Patience S Okoto, Thallapuranam K S Kumar, Paul D Adams

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Djamali MuhozaSchool of Math and Natural Sciences, University of Arkansas at Monticello, Monticello, AR, 71655, USA; Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, 72701, USA.
Emily P EsquivelSchool of Math and Natural Sciences, University of Arkansas at Monticello, Monticello, AR, 71655, USA.
Stacy R HunterSchool of Math and Natural Sciences, University of Arkansas at Monticello, Monticello, AR, 71655, USA.
Patience S OkotoDepartment of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, 72701, USA.
Thallapuranam K S KumarDepartment of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, 72701, USA.
Paul D AdamsDepartment of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, 72701, USA. Electronic address: pxa001@uark.edu.

Funding

Understanding Hesitant AdoptersP20GM103429 · NIGMS · UNIV OF ARKANSAS FOR MED SCIS · PI Lawrence E Cornett · 2012 to 2026
$60.9M
Unraveling Gene-Environment Interactions Shaping Metabolism: A Multi-Omics Analysis in DrosophilaP20GM139768 · NIGMS · UNIVERSITY OF ARKANSAS AT FAYETTEVILLE · PI Joanna Fiddler · 2021 to 2026
$17.0M
Center for Protein Structure and FunctionP30GM103450 · NIGMS · UNIVERSITY OF ARKANSAS AT FAYETTEVILLE · PI MILLETT, FRANCIS S · 2012 to 2014
$3.2M
NIGMS NIH HHS P20 GM103429NIGMS NIH HHS P20 GM139768NIGMS NIH HHS P30 GM103450
6 · The paper itself

Abstract

The serine/threonine kinase PAK1 serves as a mediator of cytoskeletal reorganization and cancer-related signaling downstream of the small GTPases. Due to the challenges in purifying PAK1 complexes, a 46-residue peptide from PAK1, is widely used to study PAK1-Cdc42 signaling. Traditionally, this purification involved multi-step chromatography of recombinant GST-PBD46 complexes, yielding approximately 1 mg per 1.5 L culture. In this study, a 30 min heat treatment step after thrombin cleavage was used to precipitate GST while leaving pure PBD46 in solution. This step eliminated the need for further affinity and size-exclusion chromatography steps. This improved protocol produces proteins with a 6.5-fold higher yield, halves the purification processing time, and produces peptides with ≥95 % purity. Mass spectrometry, CD, fluorescence, and

Indexed as

p21-Activated KinasesPeptidesChromatographyCircular DichroismMass SpectrometryRecombinant Proteinsp21-Activated KinasesPeptidesRecombinant Proteins

Identifiers

PMID41265809
PMCPMC13344149

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.