Evidence map›Paper›PMID 41265489›Full record

ArticleAnimal bioscience2026

Fermented soybean meal using Bacillus subtilis and Aspergillus oryzae positively enhances cecal microbial composition and broiler performance.

Novi Akhirini, Wara Pratitis Sabar Suprayogi, Pramita Nindya Saraswati, Adi Ratriyanto, Agung Irawan

Abstract read
In one paragraph

Article in Animal bioscience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Microorganisms · 2026
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Novi AkhiriniVocational Program of Animal Husbandry, Vocational School, Universitas Sebelas Maret, Surakarta, Indonesia.
Wara Pratitis Sabar SuprayogiVocational Program of Animal Husbandry, Vocational School, Universitas Sebelas Maret, Surakarta, Indonesia.
Pramita Nindya SaraswatiVocational Program of Animal Husbandry, Vocational School, Universitas Sebelas Maret, Surakarta, Indonesia.
Adi RatriyantoDepartment of Animal Science, Faculty of Animal Science, Universitas Sebelas Maret, Surakarta, Indonesia.
Agung IrawanVocational Program of Animal Husbandry, Vocational School, Universitas Sebelas Maret, Surakarta, Indonesia.

Funding

Institute of Research and Community Service of Universitas Sebelas Maret 371/UN27.22/PT.01.03/2025
6 · The paper itself

Abstract

objectiveThis study investigated the effect of fermented soybean meal (FSBM) prepared through solid-state fermentation using Bacillus subtilis (BS) and Aspergillus oryzae (AO) to replace SBM in broiler chickens' diets on production, digestibility, and cecal microbial profile.

methodsIn total, 160 sex-mixed day-old chicks of Cobb 500 broiler chickens were randomly assigned to four groups, four pens (replicates; 10 birds each pen), and were raised for 35 days under tropical conditions. The treatments were control (basal diet; CON) or SBM replaced by FSBM produced using AO (AO group), BS (BS group), and their combination (AO+BS group).

resultsBirds fed the AO+BS diet resulted in higher (p = 0.003) body weight (BW) while the BS diet tended (p = 0.063) to have higher final BW than CON or AO. Similarly, birds fed FSBM prepared under either BS or AO+BS had higher feed intake (p<0.01) than the CON group. No difference was found in feed conversion ratio. Relative organ weights including heart, liver, abdominal fat, and total inner organs were lower (p<0.01) in birds fed the AO+BS diet than the CON group, but relative carcass weight was unaffected. Treatments with BS or AO+BS increased dry matter (DM; p = 0.032), organic matter (p = 0.016), and crude protein (CP; p = 0.044) digestibility, while AO did not affect DM and CP digestibility. Broilers fed the AO+BS diet showed greater abundance of Firmicutes phylum and Bacteroides genus than the CON group. Several microbial taxa biomarkers were identified via linear discriminant analysis effect size analysis, including higher abundance of Enterococcus and Bacillus in the AO+BS group but lower abundance of Erysipelatoclostridium, Odoribacter, Ruminococcaceae bacterium, Staphylococcus, and Clostridium methylpentosum group in the CON group.

conclusionB. subtilis and A. oryzae could synergistically enhance the nutritional quality of SBM, positively alter cecal microbiota, and improve the production performance of broiler chickens.

Indexed as

Antinutritional FactorMicrobiomePoultrySolid-state Fermentation

Identifiers

PMID41265489
PMCPMC13064996

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.