ArticleEnvironmental analysis, health and toxicology2025
Development of an integrated testing strategy using in vitro models to predict lung carcinogenesis.
Article in Environmental analysis, health and toxicology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- An AOP-Based Integrated In Vitro and In Vivo Assessment of the Non-Genotoxic Carcinogenic Potential of Multi-Walled Carbon Nanotubes.Nanomaterials (Basel, Switzerland) · 2026Article
- Four Decades of Environmental Analysis, Health and Toxicology.Environmental analysis, health and toxicology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Carcinogenicity testing has traditionally been conducted using long-term animal studies, as specified in OECD TG 451 and 453 guidelines. These studies typically use rats and last for two years, requiring significant time and resources. Consequently, there is a pressing need to develop alternative toxicity testing methods that can efficiently predict lung cancer risks caused by chronic chemical exposure. In this study, we designed integrated testing strategies (ITS) to assess carcinogenesis by focusing on cell survival, clonal growth, and metastasis using the BEAS-2B cell model. Non-tumorigenic BEAS-2B cells were exposed to Benzo(a)pyrene (B(a)P), Ethyl carbamate (EC), Epichlorohydrin (ECH), and chloromethyl methyl ether (CMME) for 4 months (#40 passages). After treatment, the BEAS-2B cells showed enhanced anchorage-dependent and anchorage-independent colony formation. Furthermore, cell migration and invasion assays using transwell chambers revealed a significant increase in these malignant characteristics in treated BEAS-2B cells. Collectively, our findings demonstrate that prolonged exposure of non-tumorigenic BEAS-2B cells to B(a)P, EC, ECH, and CMME can lead to the acquisition of metastatic potential and multiple malignant characteristics. These integrated testing strategies for assessing carcinogenic potential could serve as a valuable tool for identifying unknown carcinogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.