ArticleProceedings of the National Academy of Sciences of the United States of America2025
Genome integrity relies on rapid recycling of DNA Pol III in bacteria.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Microbial Natural Products Targeting theMicroorganisms · 2026Review
- Mechanisms of chromosomal DNA replication in Escherichia coli and Bacillus subtilis.FEMS microbiology reviews · 2026Review
- Genome integrity relies on rapid recycling of DNA Pol III in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
DNA replication requires precise coordination between DNA unwinding and DNA synthesis. In all domains of life, protein-protein interactions at the replisome maintain proximity between the enzymes that catalyze these two activities. Surprisingly, in bacteria, the replicative DNA polymerase (Pol III), responsible for DNA synthesis, is exchanged every few seconds. How processive synthesis is maintained under these conditions has remained unclear. Here, we use single-molecule microscopy in live cells to show that Pol III rapidly rebinds the replisome within seconds of dissociation. This fast recruitment is driven by an interaction with single-stranded DNA-binding protein (SSB), which enhances Pol III target search efficiency by ~20-fold. Disrupting this mechanism alters replisome stoichiometry, causes single-stranded DNA (ssDNA) accumulation, depletes free SSB, and leads to helicase disassembly and DNA breakage, culminating in genome instability. Our findings reveal a mechanism by which fast polymerase recycling sustains continuous DNA replication and genome integrity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.