ArticleMicroscopy research and technique2026
Method for Lignin Analysis in Wood by Fluorescence Microscopy.
Article in Microscopy research and technique, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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1 citing paper in PubMed.
- Method for Lignin Analysis in Wood by Fluorescence Microscopy.Microscopy research and technique · 2026Article
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3 authors.
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Abstract
Native Amazonian species present high anatomical variation which is reflected in their lignin content. This study tested different lignin fluorescence treatments and fluorescence intensity processing methods in native Amazonian woods. Validating the fluorescence technique for lignin analysis included relating the total wood lignin content to the fluorescence intensity emitted by lignin in histological sections. Seven native Amazonian species were analyzed. Wood lignin content was obtained by the Klason method. Four treatments were used for fluorescence in the histological sections: autofluorescence (in natura and without extractives), basic fuchsin and Mäule. Images obtained with the fluorescence microscope were processed using ImageJ, applying three different methodologies to obtain fluorescence intensity-two using an integrated density formula and one automatically. Autofluorescence was the most effective treatment for relating fluorescence intensity and lignin content on a slide without extractive, especially when analyzed automatically (Method 3). It showed the second highest coefficient of determination (R
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