Evidence map›Paper›PMID 41263328›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Genome-Wide Association Analyses Reveal the Genetic Basis of EMS Mutagenesis Efficiency in Rice.

Peizhou Xu, Qinglu Zhang, Le Xue, Zhen Zhang, Nanhui Luo, Zhaoyang Cheng, Duo Xia, Shuqin Zheng, Xianjun Wu, Hao Zhou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Peizhou XuState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Qinglu ZhangNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China.
Le XueState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Zhen ZhangState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Nanhui LuoState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Zhaoyang ChengState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Duo XiaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Shuqin ZhengState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Xianjun WuState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Hao ZhouState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.ORCID https://orcid.org/0000-0003-4803-4990

Funding

National Natural Science Foundation of China 32201879National Natural Science Foundation of China 32572345Sichuan Science and Technology Program 2023NSFSC1933,2022ZDZX0012
6 · The paper itself

Abstract

Ethyl methanesulfonate (EMS) mutagenesis is widely used to generate genetic diversity in crops, but its efficiency is strongly genotype-dependent, and which underlying mechanisms remain poorly understood. Here, a large-scale phenotypic analysis of 420 diverse accessions of rice (Oryza sativa L.) is performed, revealing extensive variation in EMS tolerance (survival rate) and mutagenesis efficiency (mutation frequency). The Aus subpopulation consistently outperformed others for both traits. A genome-wide association study (GWAS) identified a major locus, SRD7, linked to reduced survival rate under EMS stress. Within this locus, the Rc gene, a key regulator of proanthocyanidins biosynthesis, is identified as the candidate causal factor. Haplotype analysis showed that functional Rc alleles confer high EMS tolerance, a conclusion further validated using transgenic knockout lines. Using near-isogenic lines (NILs), it is confirmed that Rc not only improves seed survival after EMS treatment, but also unexpectedly increases genome-wide mutation frequency. Mechanistic studies demonstrated that Rc enhances the antioxidant capacity of seeds by elevating CAT, SOD, and POD activities, while reducing H

Indexed as

Ethyl MethanesulfonateMutagenesisOryzaGenome-Wide Association StudyPlant ProteinsEthyl MethanesulfonatePlant Proteinsantioxidant capacityEMS mutagenesismutation breedingoryza sativaRc gene

Identifiers

PMID41263328
PMCPMC12884769

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.