Evidence map›Paper›PMID 41262570›Full record

ArticleACS pharmacology & translational science2025

A Novel Fluorescence Polarization Binding Assay for the Main Protease (M

Mackenzie K Wyllie, Rayhan G Biswas, Jyoti Vishwakarma, Morgan A Esler, Joseph A Rollie, Hideki Aihara, Reuben S Harris, Daniel A Harki

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS pharmacology & translational science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mackenzie K WyllieDepartment of Medicinal Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, United States.
Rayhan G BiswasDepartment of Medicinal Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, United States.
Jyoti VishwakarmaDepartment of Biochemistry and Structural Biology, University of Texas Health San Antonio, San Antonio, Texas 78229, United States.
Morgan A EslerDepartment of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455, United States.
Joseph A RollieDepartment of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455, United States.
Hideki AiharaDepartment of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455, United States.ORCID https://orcid.org/0000-0001-7508-6230
Reuben S HarrisDepartment of Biochemistry and Structural Biology, University of Texas Health San Antonio, San Antonio, Texas 78229, United States.
Daniel A HarkiDepartment of Medicinal Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, United States.ORCID https://orcid.org/0000-0001-5950-931X

Funding

Project 5: Pandemic Virus Helicase InhibitorsU19AI171954 · NIAID · UNIVERSITY OF MINNESOTA · PI MING LUO · 2022 to 2026
$100.9M
Women's CancerP30CA077598 · NCI · UNIVERSITY OF MINNESOTA TWIN CITIES · PI Douglas Yee · 1998 to 2026
$100.4M
Structural studies of viral replication and invasionR35GM118047 · NIGMS · UNIVERSITY OF MINNESOTA · PI Hideki Aihara · 2016 to 2026
$6.3M
NCI NIH HHS P30 CA077598NIAID NIH HHS U19 AI171954NIGMS NIH HHS R35 GM118047
6 · The paper itself

Abstract

The COVID-19 pandemic incited a global health crisis that accelerated the development of antiviral therapeutics. One successful avenue for inhibiting SARS-CoV-2 has been through targeting the main protease (M

Indexed as

binding assayfluorescence polarizationmain proteaseSARS-CoV-2screening

Identifiers

PMID41262570
PMCPMC12624486

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.