Evidence map›Paper›PMID 41261881›Full record

ArticleAngewandte Chemie (International ed. in English)2026

Long Single-Stranded DNA with Site-Specific Modifications to Enable Single-Base-Resolution Epigenetics.

Chenyou Zhu, Yin Xu, Ziyang Hao, Yuan Zhuang, Rui Xu, Yufan Pan, Jiezhong Shi, Yujie Li, Yifan Jiang, Tianran Wang and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Chenyou ZhuEngineering Research Center of Advanced Rare Earth Materials (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084, P.R. China.
Yin XuChina National Center for Bioinformation, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100101, P.R. China.
Ziyang HaoSchool of Pharmaceutical Sciences, Capital Medical University, Beijing, 100069, P.R. China.
Yuan ZhuangEngineering Research Center of Advanced Rare Earth Materials (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084, P.R. China.
Rui XuEngineering Research Center of Advanced Rare Earth Materials (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084, P.R. China.
Yufan PanEngineering Research Center of Advanced Rare Earth Materials (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084, P.R. China.
Jiezhong ShiEngineering Research Center of Advanced Rare Earth Materials (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084, P.R. China.
Yujie LiEngineering Research Center of Advanced Rare Earth Materials (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084, P.R. China.
Yifan JiangEngineering Research Center of Advanced Rare Earth Materials (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084, P.R. China.
Tianran WangSchool of Pharmaceutical Sciences, Capital Medical University, Beijing, 100069, P.R. China.
Yuhe R YangCAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, P.R. China.
Huajie LiuSchool of Chemical Science and Engineering, Shanghai Research Institute for Intelligent Autonomous Systems, Key Laboratory of Advanced Civil Engineering Materials of Ministry of Education, Tongji University, Shanghai, 200092, P.R. China.
Dali HanChina National Center for Bioinformation, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100101, P.R. China.
Yuanchen DongCAS Key Laboratory of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun Beiyijie, Beijing, 100190, P.R. China.
Chuan HeDepartment of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL, 60637, USA.
Dongsheng LiuEngineering Research Center of Advanced Rare Earth Materials (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084, P.R. China.ORCID 0000-0002-2583-818X

Funding

Beijing Natural Science Foundation JQ24007Howard Hughes Medical InstituteNational Basic Research Plan of China 2023YFA0915201National Basic Research Plan of China 2024YFA1308500National Natural Science Foundation of China 22477122National Natural Science Foundation of China 32270627Open Project of State Key Laboratory of Supramolecular Structure and Materials sklssm202506Strategic Priority Research Program of the Chinese Academy of Sciences XDB0960000
6 · The paper itself

Abstract

We report a method for synthesizing site-specifically modified long single-stranded DNA (ssDNA) with high purity and fidelity. With the successful synthesis of long ssDNA carrying different 5-methylcytosine (5mC) patterns, single-base-resolution analysis of 5mC epigenetic dynamics at specific genomic loci were enabled in mammalian cells. The results revealed that the density of 5mC sites synergistically correlated with their maintenance. This study opens new avenues for precise epigenetic manipulation and therapeutic applications.

Indexed as

DNA, Single-StrandedEpigenesis, Genetic5-MethylcytosineDNA MethylationHumans5-MethylcytosineDNA, Single-Stranded5‐Methyl cytosineEpigeneticsLong single‐stranded DNASingle‐base resolutionSite‐specific modifications

Identifiers

PMID41261881
PMCPMC13602406

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.