Evidence map›Paper›PMID 41258312›Full record

ArticleCurrent medical science2025

PPA1 Facilitates Thermogenesis in Brown and Beige Fat by Regulating the Mitochondrial Localization of FUS.

Yue Sun, Heng-le Ding, Jin-Fu Zhang, Yuan-Yuan Su, Nan Yang, Ye Yin, Hai-Yan Lin, Xiao-Rong Zhu

Abstract read
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In one paragraph

Article in Current medical science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yue SunDepartment of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210000, China.ORCID http://orcid.org/0009-0002-7575-5312
Heng-le DingThe Key Laboratory of the Molecular Targets of Metabolic Diseases and Intervention of Jiangsu Province, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, 210000, China.
Jin-Fu ZhangThe Key Laboratory of the Molecular Targets of Metabolic Diseases and Intervention of Jiangsu Province, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, 210000, China.
Yuan-Yuan SuThe Key Laboratory of the Molecular Targets of Metabolic Diseases and Intervention of Jiangsu Province, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, 210000, China.
Nan YangThe Key Laboratory of the Molecular Targets of Metabolic Diseases and Intervention of Jiangsu Province, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, 210000, China.
Ye YinThe Key Laboratory of the Molecular Targets of Metabolic Diseases and Intervention of Jiangsu Province, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, 210000, China. yin@njmu.edu.cn.
Hai-Yan LinThe Key Laboratory of the Molecular Targets of Metabolic Diseases and Intervention of Jiangsu Province, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, 210000, China. linda@njmu.edu.cn.
Xiao-Rong ZhuDepartment of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China. zhuxr_06@sina.com.

Funding

Capital Medical University PYZ24119National Natural Science Foundation of China 82270874National Natural Science Foundation of China 82370855
6 · The paper itself

Abstract

objectiveBrown and beige adipocytes dissipate energy through thermogenesis, and the impaired thermogenic function of these adipocytes is a key driver of obesity and related metabolic disorders. However, the molecular mechanisms governing adipocyte thermogenesis are not fully understood. This study investigated the role of inorganic pyrophosphatase 1 (PPA1) in regulating adipocyte thermogenesis and assessed its potential as a therapeutic target for obesity and metabolic disorders.

methodsTo investigate the function of PPA1 in adipose tissue thermogenesis, we generated adipose-specific heterozygous PPA1 knockout mice via the Cre-loxP system and established cold exposure models. PPA1 deletion effects were assessed by hematoxylin and eosin (H&E) staining, immunofluorescence, quantitative polymerase chain reaction (qPCR), and immunoblotting. Mitochondrial changes during browning were further characterized via transmission electron microscopy (TEM), mitochondrial DNA (mtDNA) quantification, qPCR, and Western blotting. The molecular mechanisms involved were subsequently dissected via mass spectrometry, coimmunoprecipitation (Co-IP), and immunofluorescence colocalization.

resultsAdi-PPA1

conclusionPPA1 functions as a nonenzymatic positive regulator of adipocyte thermogenesis by interacting with FUS to prevent its mitochondrial mislocalization, thereby maintaining mitochondrial function and promoting adipose browning. These findings highlight PPA1 as a potential therapeutic avenue for obesity and metabolic disorders.

Indexed as

Adipose Tissue, BeigeAdipose Tissue, BrownMitochondriaThermogenesisAnimalsMaleMiceMice, KnockoutObesityAdipose browningFUSMitochondriaObesityPPA1Thermogenesis

Identifiers

PMID41258312

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.