ArticleScientific reports2025
Production of offspring via the transplantation of frozen germ cells from Tokyo bitterling, a fish on the brink of extinction.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Efficient recovery of zebrafish maternal-zygotic mutants from in vivo expanded and cryopreserved germline stem cells.Communications biology · 2026Article
- Germ cell manipulation in farmed fishes.The Journal of reproduction and development · 2026Review
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Authors and funding
3 authors.
Funding
Abstract
Many freshwater fish face extinctions due to habitat destruction and overfishing. Bitterlings, which lay their eggs in the gill chambers of freshwater mussels, are in steep decline worldwide, along with mussels, owing to the concreting and siltation of riverbeds. In particular, Tokyo bitterling is listed as "endangered" on the Red List and is on the verge of extinction. Although in situ and ex situ conservation efforts are underway, their effectiveness is limited. Here, we developed a method for long-term preservation of genetic resources by combining germ cell cryopreservation and transplantation. We transplanted Tokyo bitterling germ cells stored in liquid nitrogen into oily bitterling recipients lacking endogenous germ cells due to dnd knockdown, and the recipients matured and repeatedly produced many eggs and sperm. The resulting offspring had the Tokyo bitterling genotype and morphology. This study shows that even extinct species can be revived if their germ cell bank is available.
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