Evidence map›Paper›PMID 41258013›Full record

ArticleNature communications2025

Experimental phage evolution results in expanded host ranges against antibiotic resistant Klebsiella pneumoniae isolates.

Pooja Ghatbale, Alisha Blanc, Andrew Sue, Jesse Leonard, Monica Bates, Andrew G Garcia, Joshua Hensley, Danielle Devequi Gomes Nunes, Nicole Hitchcock, Job Shiach and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Antibiotic resistance inFrontiers in microbiology · 2026
    Review
  6. Frontiers in microbiology · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Pooja GhatbaleDepartment of Pathology, University of California, San Diego, CA, USA.
Alisha BlancDepartment of Pathology, University of California, San Diego, CA, USA.
Andrew SueDepartment of Pathology, University of California, San Diego, CA, USA.
Jesse LeonardDepartment of Pathology, University of California, San Diego, CA, USA.
Monica BatesDepartment of Pathology, University of California, San Diego, CA, USA.
Andrew G GarciaDepartment of Pathology, University of California, San Diego, CA, USA.
Joshua HensleyNorth Carolina Research Center, Department of Bioinformatics and Genomics, The University of North Carolina at Charlotte, Kannapolis, NC, USA.
Danielle Devequi Gomes NunesSENAI Institute Innovation (ISI) in Health Advanced System, University Center SENAI/ CIMATEC, Salvador, Bahia, Brazil.
Nicole HitchcockDepartment of Pathology, University of California, San Diego, CA, USA.
Job ShiachDepartment of Medicine, University of California, San Diego, CA, USA.ORCID http://orcid.org/0000-0002-6518-9663
Roberto BardaróSENAI Institute Innovation (ISI) in Health Advanced System, University Center SENAI/ CIMATEC, Salvador, Bahia, Brazil.
Govind SahDepartment of Pathology, University of California, San Diego, CA, USA.
Chandrabali GhoseBioharmony, Inc., New York, NY, USA.
Katrine L WhitesonDepartment of Biology, University of California, Irvine, CA, USA.ORCID http://orcid.org/0000-0002-5423-6014
Robert T SchooleyDepartment of Medicine, University of California, San Diego, CA, USA.ORCID http://orcid.org/0000-0002-2498-8426
Richard Allen WhiteNorth Carolina Research Center, Department of Bioinformatics and Genomics, The University of North Carolina at Charlotte, Kannapolis, NC, USA.
Ana G Cobián GüemesDepartment of Pathology, University of California, San Diego, CA, USA.
Justin R MeyerDepartment of Ecology, Behavior and Evolution, University of California, San Diego, CA, USA.ORCID http://orcid.org/0000-0001-5566-8452
David T PrideDepartment of Pathology, University of California, San Diego, CA, USA. dpride@health.ucsd.edu.

Funding

Illumina NovaSeq 6000 Sequencing SystemS10OD026929 · OD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI JEPSEN, KRISTEN LYNN · 2019 to 2019
$600k
NIH HHS S10 OD026929
6 · The paper itself

Abstract

Resistance to antibiotics is approaching crisis levels for organisms such as the ESKAPEE pathogens (includes Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp., and Escherichia coli) that often are acquired in hospitals. These organisms sometimes have acquired plasmids that confer resistance to most if not all beta-lactam antibiotics. We have been developing alternative means for dealing with antibiotic resistant microbes that cause infections in humans by developing viruses (bacteriophages) that attack and kill them. One of these pathogens, K. pneumoniae, has one of the highest propensities for antimicrobial resistance. We identified many phages that have lytic capacity against limited numbers of clinical isolates, and through experimental evolution over the course of 30 days, were able to vastly expand the host ranges of these phages to kill a broader range of clinical K. pneumoniae isolates including MDR (multi-drug resistant) and XDR (extensively-drug resistant) isolates. Most interestingly, they were capable of inhibiting growth of clinical isolates both on solid and in liquid medium over extended periods. That we were able to extend the host ranges of multiple naïve antibiotic resistant K. pneumoniae through experimental phage evolution suggests that such a technique may be applicable to other antibiotic-resistant organisms to help stem the tide of antibiotic resistance and offer further options for medical treatments.

Indexed as

BacteriophagesDrug Resistance, BacterialHost SpecificityKlebsiella pneumoniaeAnti-Bacterial AgentsDrug Resistance, Multiple, BacterialHumansKlebsiella InfectionsMicrobial Sensitivity TestsAnti-Bacterial Agents

Identifiers

PMID41258013
PMCPMC12630596

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.