Evidence map›Paper›PMID 41257862›Full record

ArticleCancer cell international2025

Multi-omics analysis unveils the role of cancer-associated fibroblasts in cutaneous squamous cell carcinoma.

Xiaochuan Wang, Tingrui Li, Yichao Jin, Jingjing Chen, XinYang, Zhen Guan, Mei Jin, Jingxian Zhang, Liangheng Xu, Sizhen Tao and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer cell international, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Xiaochuan Wang *Department of Dermatology, the First People's Hospital of Yunnan Province, Yunnan Provincial Key Laboratory of Clinical Virology, The Affiliated Hospital of Kunming University of Science and Technology, 157 Jinbi Lu, Kunming, 650032, Yunnan, P.R. China.
Tingrui Li *Department of Dermatology, the First People's Hospital of Yunnan Province, Yunnan Provincial Key Laboratory of Clinical Virology, The Affiliated Hospital of Kunming University of Science and Technology, 157 Jinbi Lu, Kunming, 650032, Yunnan, P.R. China.
Yichao JinDepartment of Dermatology, the First People's Hospital of Yunnan Province, Yunnan Provincial Key Laboratory of Clinical Virology, The Affiliated Hospital of Kunming University of Science and Technology, 157 Jinbi Lu, Kunming, 650032, Yunnan, P.R. China.
Jingjing ChenDepartment of Dermatology, the First People's Hospital of Yunnan Province, Yunnan Provincial Key Laboratory of Clinical Virology, The Affiliated Hospital of Kunming University of Science and Technology, 157 Jinbi Lu, Kunming, 650032, Yunnan, P.R. China.
XinYangDepartment of Dermatology, the First People's Hospital of Yunnan Province, Yunnan Provincial Key Laboratory of Clinical Virology, The Affiliated Hospital of Kunming University of Science and Technology, 157 Jinbi Lu, Kunming, 650032, Yunnan, P.R. China.
Zhen GuanDepartment of Dermatology, the First People's Hospital of Yunnan Province, Yunnan Provincial Key Laboratory of Clinical Virology, The Affiliated Hospital of Kunming University of Science and Technology, 157 Jinbi Lu, Kunming, 650032, Yunnan, P.R. China.
Mei JinDepartment of Dermatology, the First People's Hospital of Yunnan Province, Yunnan Provincial Key Laboratory of Clinical Virology, The Affiliated Hospital of Kunming University of Science and Technology, 157 Jinbi Lu, Kunming, 650032, Yunnan, P.R. China.
Jingxian ZhangDepartment of Dermatology, the First People's Hospital of Yunnan Province, Yunnan Provincial Key Laboratory of Clinical Virology, The Affiliated Hospital of Kunming University of Science and Technology, 157 Jinbi Lu, Kunming, 650032, Yunnan, P.R. China.
Liangheng XuDepartment of Dermatology, the First People's Hospital of Yunnan Province, Yunnan Provincial Key Laboratory of Clinical Virology, The Affiliated Hospital of Kunming University of Science and Technology, 157 Jinbi Lu, Kunming, 650032, Yunnan, P.R. China.
Sizhen TaoDepartment of Dermatology, the First People's Hospital of Yunnan Province, Yunnan Provincial Key Laboratory of Clinical Virology, The Affiliated Hospital of Kunming University of Science and Technology, 157 Jinbi Lu, Kunming, 650032, Yunnan, P.R. China.
Chunguang LiDepartment of Dermatology, the People's Hospital of Mengzi, 89 Tianma Lu, Mengzi, 661100, Yunnan, P.R. China. 893907947@qq.com.
Chunping AoDepartment of Dermatology, the First People's Hospital of Yunnan Province, Yunnan Provincial Key Laboratory of Clinical Virology, The Affiliated Hospital of Kunming University of Science and Technology, 157 Jinbi Lu, Kunming, 650032, Yunnan, P.R. China. aochunping11@163.com.

Funding

Yunnan Fundamental Research Projects 202401AT070051Yunnan Provincial Department of Science and Technology-Kunming Medical University Joint Special Project on Applied Basic Research 202201AY070001-251Yunnan Provincial Key Laboratory of Clinical Virology Open Project 202205AG070061-BD-02
6 · The paper itself

Abstract

backgroundHuman papillomavirus (HPV) infection is associated with an increased risk of cutaneous squamous cell carcinoma (CSCC). A comprehensive understanding of the cellular heterogeneity of HPV-positive and -negative CSCC is crucial for improving diagnosis and preventing tumor progression.

methodsWe conducted an integrated analysis of single-cell RNA and spatial transcriptomic data from different skin tissue sources to map the cellular landscape of the tumor microenvironment (TME) in both HPV positive and negative CSCC. Results were validated through multiplex immunohistochemistry (mIHC) and in vitro experiments.

resultsWe identified 10 major cell types in CSCC and normal skin samples, including epithelial cells, myeloid cells, T cells, fibroblasts, endothelial cells, B cells, smooth muscle cells, mast cells, melanocytes, and hair follicle cells. Notably, fibroblasts were found to be associated with tumor progression in CSCC with or without HPV infected. We further identified eight major CAF subtypes in CSCC, with iCAFs-CXCL2 promoting tumor progression, while iCAFs-PLA2G2A acted to suppress tumor growth. The MDK-ITGA6 pair was found to mediate interactions between fibroblasts and epithelial cells in CSCC. mIHC analysis confirmed elevated expression of MDK and ITGA6 in CSCC samples. Additionally, cell co-culture experiments confirmed that MDK-mediated CAFs were shown to enhance tumor cell migration and invasion in CSCC.

conclusionOur findings provide a comprehensive cellular atlas of CSCC, highlighting the association of CAFs in HPV infection and tumor progression of CSCC. These results also offer potential diagnostic and prognostic biomarkers for CSCC patients.

Indexed as

Cancer-associated fibroblastsCutaneous squamous cell carcinomaHuman papillomavirusesMulti-omics analysisMultiplex immunohistochemistry analysis

Identifiers

PMID41257862
PMCPMC12628552

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