Evidence map›Paper›PMID 41257294›Full record

ArticleFrontiers in microbiology2025

An inter-laboratory study characterizes the impact of bioinformatic approaches on genome-based cluster detection for foodborne bacterial pathogens.

Leonie F Forth, Burkhard Malorny, Markus Bönn, Erik Brinks, Grégoire Denay, Carlus Deneke, Hosny El-Adawy, Jennie Fischer, Jannika Fuchs, Ekkehard Hiller and 13 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors.

Leonie F ForthDepartment of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
Burkhard MalornyDepartment of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
Markus BönnState Office for Consumer Protection of Sachsen-Anhalt, Halle (Saale), Germany.
Erik BrinksInstitute of Microbiology and Biotechnology, Max Rubner-Institut, Kiel, Germany.
Grégoire DenayChemical and Veterinary Analytical Institute Rhein-Ruhr-Wupper (CVUA-RRW), Krefeld, Germany.
Carlus DenekeDepartment of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
Hosny El-AdawyInstitute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, Jena, Germany.
Jennie FischerDepartment of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
Jannika FuchsChemical and Veterinary Analysis Agency Karlsruhe, Karlsruhe, Germany.
Ekkehard HillerChemical and Veterinary Analysis Agency Stuttgart, Fellbach, Germany.
Nancy BretschneiderBavarian Health and Food Safety Authority, Oberschleißheim, Germany.
Sylvia KletaDepartment of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
Stefanie LüthDepartment of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
Tilman SchultzeDepartment of Veterinary Medicine, Hessian State Laboratory, Giessen, Germany.
Henning PetersenChemical and Veterinary Analytical Institute Ostwestfalen-Lippe, Detmold, Germany.
Michaela ProjahnDepartment of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
Christian SchäfersHamburg Public Laboratories for Food Safety, Health Protection and Environmental Assessment, Institute for Hygiene and Environment, Hamburg, Germany.
Kerstin StinglDepartment of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
Andreas J StroehleinDepartment of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
Laura UelzeSequencing and Genotyping Service Unit, Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany.
Kathrin SzaboDepartment Official Collection of Methods of Analysis (ASU), Federal Office of Consumer Protection and Food Safety, Berlin, Germany.
Anne WöhlkeFood and Veterinary Institute Braunschweig/Hannover, Lower Saxony State Office for Consumer Protection and Food Safety, Braunschweig, Germany.
Jörg LindeInstitute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, Jena, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Accurate assignment of whole-genome sequences to clusters in foodborne outbreak investigations remains challenging. Variability in bioinformatics tools and quality metrics significantly impacts clustering outcomes. This study assessed inter-laboratory variance in cluster identification by providing four datasets of 50 raw Illumina paired-end sequences covering Shiga toxin-producing

Indexed as

bioinformatic toolscluster detectionfoodborne pathogensfood safetyinter-laboratory studypublic healthwhole-genome sequencing

Identifiers

PMID41257294
PMCPMC12621568

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.