Evidence map›Paper›PMID 41257250›Full record

ArticleNAR molecular medicine2024

A chimeric RNA consisting of siRNA and aptamer for inhibiting dengue virus replication.

Ryo Amano, Masaki Takahashi, Kazumi Haga, Mizuki Yamamoto, Kaku Goto, Akiko Ichinose, Michiaki Hamada, Jin Gohda, Jun-Ichiro Inoue, Yasushi Kawaguchi and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in NAR molecular medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Ryo AmanoProject Division of RNA Medical Science, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Masaki TakahashiProject Division of RNA Medical Science, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Kazumi HagaDepartment of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Mizuki YamamotoResearch Center for Asian Infectious Diseases, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Kaku GotoProject Division of RNA Medical Science, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Akiko IchinoseGraduate School of Advanced Science and Engineering, Waseda University, 3-4-1, Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
Michiaki HamadaGraduate School of Advanced Science and Engineering, Waseda University, 3-4-1, Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.ORCID https://orcid.org/0000-0001-9466-1034
Jin GohdaResearch Center for Asian Infectious Diseases, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Jun-Ichiro InoueThe University of Tokyo Pandemic Preparedness, Infection and Advanced Research Center (UTOPIA), 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Yasushi KawaguchiResearch Center for Asian Infectious Diseases, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Meng Ling MoiDepartment of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Yoshikazu NakamuraProject Division of RNA Medical Science, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Silencing viruses by chimeric RNAs, wherein small interfering RNAs (siRNAs) targeting viral RNAs are conjugated with RNA aptamers specific to viral envelope proteins, is a promising treatment for viral infection diseases; however, practical evaluations are apparently lacking. Here, we present a chimeric RNA comprises siRNA and RNA aptamer, both of which target all four serotypes of dengue virus (DENV), for suppressing DENV replication. The siRNA targeting consensus sequences in the 3'-UTR of all four DENV serotypes suppressed the expression of a reporter gene carrying the siRNA-targeted sequence of DENV-1 by ∼70%. The RNA aptamer generated by VLP-SELEX using DENV-1-VLPs as baits showed an affinity for all four DENV-VLP serotypes, presumably without affecting the fusion process. After conjugation of each modality, the chimeric RNA significantly suppressed authentic DENV-1 and DENV-2 production

Identifiers

PMID41257250
PMCPMC12429950

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.