ArticlePlant biotechnology (Tokyo, Japan)2025
CYP71D8 and CYP82A2 catalyze the last committed step in biosynthesis of glyceollin isomers in soybean.
Article in Plant biotechnology (Tokyo, Japan), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed.
- Discovery of pseudobaptigenin synthase in fungal-infected Trifolium pratense roots.The Plant journal : for cell and molecular biology · 2026Article
- Discover the maze-like network for glabridin biosynthesis.Nature communications · 2026Article
- Cytochrome P450 gene family: cross-pathway functional conservation, novel catalytic reactions, and synthetic biology-driven applications in plant secondary metabolism.Frontiers in plant science · 2026Review
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3 authors.
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Abstract
Glyceollins, which are prenylated pterocarpan phytoalexins found in soybean, play important roles in plant-microbe interactions. During biosynthesis, the formation of the cyclic ether ring from the C-5 prenyl side chain provides structural diversity to the glyceollin isomers. This reaction has been attributed to cytochrome P450 (P450); however, it is unclear whether a single enzyme or multiple enzymes are involved in glyceollin isomer formation. In this study, we searched a co-expressed gene network database for soybean. Known genes involved in glyceollin biosynthesis were used as queries, and eight P450s (CYP71D8, CYP81E24, CYP82A2, CYP82A3, CYP82A4, CYP93A2, CYP93A3, and CYP736A33) were selected as candidates. In vitro enzyme assays using recombinant yeast microsomes expressing P450s revealed that CYP71D8 produced glyceollin I from 4-dimethylallylglycinol, and CYP82A2 yielded glyceollin III from 2-dimethylallylglycinol. Real-time PCR analysis showed that transcripts of
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