ArticleIranian journal of biotechnology2025
Mathematical Modeling and Thermodynamic Integration for Precision PCR Optimization.
Article in Iranian journal of biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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4 authors.
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Abstract
Objectives: The objective of this study was to develop a predictive modeling framework for optimizing MgCl Materials and Methods: The study developed predictive models using multivariate Taylor series expansion and thermodynamic functions integrated with 120 PCR primers across various species. Ridge, Lasso, and Elastic Net multiple regression analyses were applied to fine-tune these models. Results: The models demonstrated excellent predictive capabilities, achieving an R Conclusion: The research provides a tight framework for optimizing PCR conditions and enhancing specificity and sensitivity in DNA amplification. More importantly, it is based on an approach that combines theoretical modeling with empirical validation, thus offering valuable insight into the advancement of PCR-based methodologies.
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