Evidence map›Paper›PMID 41254928›Full record

ArticleAnalytical chemistry2025

Stimulated Raman Scattering (SRS) Microscopy Enables Visualization and Quantification of Cryoprotective Agent Concentration in

Yuxiao Wei, Purva Joshi, Zachary Chau, Nora Gaby-Biegel, Leonid Peshkin, Mehmet Toner, Rebecca D Sandlin, Conor L Evans

Abstract read
In one paragraph

Article in Analytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yuxiao WeiWellman Center for Photomedicine, Massachusetts General Hospital, Charlestown, Massachusetts 02129, United States.
Purva JoshiCenter for Engineering in Medicine & Surgery, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, and Shriners Children's Boston, Boston, Massachusetts 02114, United States.ORCID 0000-0003-0362-4946
Zachary ChauCenter for Engineering in Medicine & Surgery, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, and Shriners Children's Boston, Boston, Massachusetts 02114, United States.ORCID 0009-0008-3255-0951
Nora Gaby-BiegelCenter for Engineering in Medicine & Surgery, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, and Shriners Children's Boston, Boston, Massachusetts 02114, United States.
Leonid PeshkinDepartment of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, United States.
Mehmet TonerCenter for Engineering in Medicine & Surgery, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, and Shriners Children's Boston, Boston, Massachusetts 02114, United States.
Rebecca D SandlinCenter for Engineering in Medicine & Surgery, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, and Shriners Children's Boston, Boston, Massachusetts 02114, United States.
Conor L EvansWellman Center for Photomedicine, Massachusetts General Hospital, Charlestown, Massachusetts 02129, United States.

Funding

Medical Scientist Training ProgramT32GM007753 · NIGMS · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI WALENSKY, LOREN DAVID · 1985 to 2021
$50.0M
Medical Scientist Training ProgramT32GM144273 · NIGMS · HARVARD MEDICAL SCHOOL · PI David Shumway Jones, Jacqueline A. Lees · 2022 to 2026
$14.7M
NIGMS NIH HHS T32 GM007753NIGMS NIH HHS T32 GM144273
6 · The paper itself

Abstract

Cryopreservation enables long-term storage and transport of biological specimens by minimizing or avoiding ice formation at low temperatures through the use of cryoprotective agents (CPAs). However, CPAs are often toxic at effective concentrations, necessitating precise optimization of exposure parameters such as concentration, incubation time, and temperature. Currently, the field lacks tools to monitor CPA diffusion and distribution in real time, especially in complex organisms, leading to iterative optimization that can take months to years. In this study, we present a Stimulated Raman Scattering (SRS) microscopy-based method for the spatiotemporal quantification of deuterated dimethyl sulfoxide (DMSO-

Indexed as

Cryoprotective AgentsDaphniaDimethyl SulfoxideNonlinear Optical MicroscopyAnimalsCryopreservationDaphnia magnaSpectrum Analysis, RamanCryoprotective AgentsDimethyl Sulfoxide

Identifiers

PMID41254928
PMCPMC12874216

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.