Evidence map›Paper›PMID 41254818›Full record

ArticleBiological research2025

miR-130a-3p promotes fiber type transition and improves exercise tolerance in mice.

Lipeng Xing, Hao Zhou, Haibin Deng, Binghua Yao, Junyi Luo, Ting Chen, Jiajie Sun, Songbo Wang, Gang Shu, Qingyan Jiang and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biological research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Lipeng Xing *State Key Laboratory of Livestock and Poultry Breeding, Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center For Breeding Swine Industry, Guangdong Province Research Center of Woody Forage Engineering and Technology, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou, 510642, China.ORCID http://orcid.org/0009-0002-5905-5965
Hao Zhou *State Key Laboratory of Livestock and Poultry Breeding, Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center For Breeding Swine Industry, Guangdong Province Research Center of Woody Forage Engineering and Technology, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou, 510642, China.
Haibin DengState Key Laboratory of Livestock and Poultry Breeding, Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center For Breeding Swine Industry, Guangdong Province Research Center of Woody Forage Engineering and Technology, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou, 510642, China.
Binghua YaoState Key Laboratory of Livestock and Poultry Breeding, Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center For Breeding Swine Industry, Guangdong Province Research Center of Woody Forage Engineering and Technology, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou, 510642, China.
Junyi LuoState Key Laboratory of Livestock and Poultry Breeding, Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center For Breeding Swine Industry, Guangdong Province Research Center of Woody Forage Engineering and Technology, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou, 510642, China.
Ting ChenState Key Laboratory of Livestock and Poultry Breeding, Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center For Breeding Swine Industry, Guangdong Province Research Center of Woody Forage Engineering and Technology, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou, 510642, China.
Jiajie SunState Key Laboratory of Livestock and Poultry Breeding, Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center For Breeding Swine Industry, Guangdong Province Research Center of Woody Forage Engineering and Technology, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou, 510642, China.
Songbo WangState Key Laboratory of Livestock and Poultry Breeding, Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center For Breeding Swine Industry, Guangdong Province Research Center of Woody Forage Engineering and Technology, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou, 510642, China.
Gang ShuState Key Laboratory of Livestock and Poultry Breeding, Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center For Breeding Swine Industry, Guangdong Province Research Center of Woody Forage Engineering and Technology, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou, 510642, China.
Qingyan JiangState Key Laboratory of Livestock and Poultry Breeding, Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center For Breeding Swine Industry, Guangdong Province Research Center of Woody Forage Engineering and Technology, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou, 510642, China.
Yongliang ZhangState Key Laboratory of Livestock and Poultry Breeding, Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center For Breeding Swine Industry, Guangdong Province Research Center of Woody Forage Engineering and Technology, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou, 510642, China.
Qianyun XiState Key Laboratory of Livestock and Poultry Breeding, Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center For Breeding Swine Industry, Guangdong Province Research Center of Woody Forage Engineering and Technology, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou, 510642, China. xqy0228@163.com.

Funding

National Natural Science Foundation of China 32072814Natural Science Foundation of Guangdong Province 2023A151502511
6 · The paper itself

Abstract

backgroundSkeletal muscle plays various roles in physiological stress, such as movement, hormone secretion and oxidative metabolism. MicroRNA (miRNA) is involved in skeletal muscle development and fiber type transformation. Our previous studies have shown that miR-130a-3p is an important regulator of glycolipid metabolism and participates in the aerobic oxidation process. However, its potential impact on skeletal muscle development and muscle fiber type transformation by enhancement of aerobic metabolism of nutrients remains unexplored.

methodsMice with knockout and overexpression of miR-130a-3p were utilized to investigate its impact on mouse muscle development via assessments of body composition, metabolic activity in cages, and running performance. The influence of miR-130a-3p on muscle cell proliferation, differentiation, and fiber type was assessed following transfection of C2C12 cells.

resultsIn this study, we found that miR-130a-3p overexpression significantly inhibited weight gain and fat content in mice, while promoting oxidative metabolism and improving exercise tolerance. Upregulation of miR-130a-3p in gastrocnemius (GAS) increased the expression of genes controlling cell proliferation, such as proliferating cell nuclear antigen (PCNA), cyclin D, and cyclin E, and decreased the protein expression of myosin heavy chain (MyHC), myogenic differentiation 1 (MyoD) and myopoietin (MyoG). In addition, miR-130a-3p overexpression significantly increased the proportion of type I fibers and decreased the proportion of type IIB fibers in GAS. These phenotype were also observed when miR-130a-3p was overexpressed in differentiated C2C12 myoblasts. Mechanistically, miR-130a-3p can promote the proliferation of myoblasts and inhibit the differentiation of myoblasts. Importantly, miR-130a-3p was proved to target the transforming growth factor beta receptor 2 (TGFβR2). Taken together, miR-130a-3p could be a potential therapeutic target that regulates fiber type conversion during skeletal muscle development and improves skeletal muscle energy metabolism during exercise through regulating TGFβR2 signalling pathway.

Indexed as

Exercise ToleranceMicroRNAsMuscle DevelopmentMuscle Fibers, SkeletalMuscle, SkeletalPhysical Conditioning, AnimalAnimalsCell DifferentiationCell ProliferationMaleMiceMice, KnockoutMicroRNAsMIRN130 microRNA, mouseExercise tolerancemiR-130a-3pOxidative metabolismSkeletal muscleTGFβR2

Identifiers

PMID41254818
PMCPMC12628931

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.