SynthesisEuropean journal of medical research2025
Effect of sperm DNA fragmentation on embryo euploidy rate in assisted reproductive technologies: a systematic review and meta-analysis.
Synthesis in European journal of medical research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Preimplantation genetic testing for male factor infertility: lights and shadows.Journal of endocrinological investigation · 2026Review
- Double-stranded sperm DNA fragmentation measured with neutral comet assay as a predictor of IVF outcomes: evidence from three European clinics in a multi-centred prospective study.Human reproduction (Oxford, England) · 2026Article
- High sperm DNA fragmentation is associated with increased embryonic chromosomal abnormality: a retrospective study of 8 years involving 5637 couples undergoing ART.Human reproduction open · 2026Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
objectivesTo quantitatively evaluate the association between sperm DNA fragmentation (SDF) and embryo euploidy rates in assisted reproductive technology (ART) cycles through a systematic review and meta-analysis. MATERIALS AND
methodsFollowing the PRISMA 2020 guidelines, a comprehensive search of PubMed, Web of Science, Embase, Scopus, and the Cochrane Library was conducted from inception to August 5, 2025. Eligible studies included infertile couples undergoing in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI), in which SDF was assessed using validated assays and embryo euploidy was determined via preimplantation genetic testing for aneuploidy (PGT-A). The Newcastle-Ottawa Scale was used for quality assessment. Pooled odds ratios (ORs) with 95% confidence intervals (CIs) were calculated using random- or fixed-effects models based on heterogeneity.
resultsSix studies involving 1,516 ART cycles met the inclusion criteria. All studies measured SDF using the sperm chromatin structure assay (SCSA), with cutoff values ranging from 15 to 30%. Embryo chromosomal status was evaluated at the blastocyst stage using PGT-A platforms, such as next-generation sequencing (NGS), array comparative genomic hybridization (aCGH), or single nucleotide polymorphism (SNP) arrays, with whole genome amplification (WGA) applied as a pre-analytical step rather than a detection method. Meta-analysis revealed no significant association between high SDF and embryo euploidy when using the 15% cutoff (pooled OR = 0.897; 95% CI 0.741-1.085; I
conclusionsElevated SDF, particularly above 30%, is associated with a reduced likelihood of obtaining euploid embryos in ART cycles, suggesting a potential threshold-dependent effect of sperm DNA integrity on embryo chromosomal normality. These findings support the integration of SDF assessment into the evaluation of selected couples, especially in cases of recurrent ART failure or advanced maternal age. Further prospective studies with standardized SDF protocols and uniform PGT-A methods are warranted to validate these results.
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Registered trials
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