ArticleBMC research notes2025
Molecular investigation of Sarcocystis spp., and Toxoplasma gondii in meat products in Iran.
Article in BMC research notes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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4 authors.
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Abstract
Consumption of processed meat products and fast foods are popular, all over the world. This investigation examined the prevalence of Sarcocystis spp. and Toxoplasma gondii in processed meats (hamburgers, sausages, jambon) due to their global consumption. A total of 160 samples, including hamburgers, sausages, and jambon, were collected from 15 markets (Market 1-Market 15). Conventional digestion technique was performed to isolate cysts of the parasites. Samples were stained by Giemsa and microscopically studied. Upon DNA extraction, the presence of the parasites was investigated using real-time PCR. In the present study, Sarcocystis spp., were detected in different types of meat products. The results showed that 40/160 (25%) of samples were positive for Sarcocystis spp. in 11/62 (17.7%), 11/42 (26.2%), and 18/56 (32.1%) of sausages, hamburgers, and jambon, respectively. The results of the real-time PCR presented as average cycle threshold (Ct) values for the positive samples 29.83 ± 2.7 with an average melting temperature (T
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