ArticleStem cell research & therapy2025
Mechanism of platelet-rich plasma in improving cyclophosphamide-induced premature ovarian failure in rats.
Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Platelet-rich plasma and folliculogenesis: a systematic mechanistic review and computational pathway analysis.Frontiers in reproductive health · 2026Pooled it
- Current Status of Animal Models for Premature Ovarian Insufficiency: Similarities to Clinical Manifestations and Therapeutic Applications.Tissue engineering and regenerative medicine · 2026Review
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Authors and funding
7 authors.
Funding
Abstract
backgroundPremature ovarian insufficiency (POI) is a clinical condition characterised by the cessation of ovarian function leading to infertility. Platelet-rich plasma (PRP) has attracted considerable attention in regenerative medicine. In this study, we aimed to evaluate the effectiveness of PRP in a rat model of POI induced by cyclophosphamide.
methodEighteen 8-12-week-old female rats were divided into three groups: A, control group; B, model group (induced by cyclophosphamide); and E, intervention group (induced by cyclophosphamide + PRP). At the end of the experiment, body weight; ovarian parameters; and serum levels of the sex hormones, follicle-stimulating hormone (FSH), luteinising hormone (LH), oestradiol (E2), anti-Müllerian hormone (AMH), progesterone, and testosterone, were measured. Western blotting was used to detect the expression of related proteins, such as markers for germ cells, ovarian tissue angiogenesis, and granulosa cells, as well as cellular oxidative stress levels and DNA damage repair. Serum was used in proteomic and metabolomic analyses to cluster pathway networks of differentially expressed proteins (DEPs)and metabolites and conduct further correlation analyses.
resultThe levels of FSH, LH, E2, AMH, progesterone, and testosterone improved (p < 0.05) after POI rats received the PRP intervention. PRP also significantly increased the expression levels of the germ cell markers Ddx4, PCNA, and BMP4 in the ovarian tissues of POI rats, as well as the expression levels of the ovarian tissue generation and granulosa cell markers CD34, Bcl2, Caspase3, AMH, and FSHR (p < 0.05). PRP improved oxidative stress and mitochondrial and DNA damage in the granulosa cells of rats. Proteomic and metabolomics analyses revealed energy metabolism dysfunction in rats, and the inflammatory response was significantly alleviated after PRP intervention. These results collectively indicate that PRP may act on the ovarian microenvironment through multi-target and multi-pathway approaches, thereby providing a promising strategy for the treatment of POI.
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